LOCUS Construct_10 4600 bp DNA circular UNA 04-MAY-2014 DEFINITION CMVp-mKate2_EX1-[28-gRNA1]HSV1-mKate2_EX2-pA. ACCESSION urn.local...1397744376706.306 VERSION urn.local...1397744376706.306 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(922..1782) /created_by="User" /label="AmpR" rep_origin 1914..2369 /created_by="User" /label="phage f1 origin of replication" promoter 2498..3086 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature order(3051..3057,3051..3057) /created_by="User" /label="TATA box" misc_signal 3093..3098 /created_by="User" /label="KOZAK" gene 3102..3983 /Original_Bases /modified_by="User" /label="mKate2" misc_feature 3369..3374 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /label="Donor Site" misc_feature 3375..3402 /created_by="User" /label="28nt Csy4 rpt" misc_signal 3394..3395 /created_by="User" /label="Csy4 cleaves b/w G&C" gRNA 3403..3506 /created_by="User" /modified_by="User" /label="gRNA1" misc_feature 3507..3513 /created_by="User" /label="Adeno_branch_point" misc_feature 3514..3518 /created_by="User" /label="XhoI_part" misc_feature 3519..3534 /created_by="User" /modified_by="User" /label="Poly_pirimidine tract" misc_feature 3535..3539 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /label="Acceptor\Site" polyA_signal 3990..4442 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 61 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 121 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 181 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 241 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 301 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg 361 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 421 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 481 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 541 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 601 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 661 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 721 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 781 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 841 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 901 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 961 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 1021 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 1081 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 1141 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 1201 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 1261 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 1321 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 1381 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 1441 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 1501 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 1561 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 1621 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 1681 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 1741 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 1801 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 1861 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc 1921 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 1981 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 2041 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 2101 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 2161 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 2221 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 2281 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 2341 ttttaacaaa atattaacgt ttacaatttc ccattcgcca ttcaggctgc gcaactgttg 2401 ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt 2461 aagtaatatt aaggtacggg aggtacttgg aggtacctag ttattaatag taatcaatta 2521 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 2581 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 2641 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 2701 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 2761 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 2821 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2881 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2941 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 3001 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 3061 gagctggttt atgaaccgtc agatccgagc tcgccaccat ggtgtctaag ggcgaagagc 3121 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact 3181 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca 3241 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt 3301 acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct 3361 tccctgaggt aagtgttcac tgccgtatag gcagctaaga aatagtcgcg tgtagcgaag 3421 cagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa 3481 gtggcaccga gtcggtgctt tttttcgagg gagtcgagtc ttcttttttt ttttcacagg 3541 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg 3601 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc 3661 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc accgagatgc 3721 tgtaccccgc tgacggcggc ctggaaggca gaagcgacat ggccctgaag ctcgtgggcg 3781 ggggccacct gatctgcaac ttgaagacca catacagatc caagaaaccc gctaagaacc 3841 tcaagatgcc cggcgtctac tatgtggaca gaagactgga aagaatcaag gaggccgaca 3901 aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca 3961 aactggggca caaacttaat tgacccgggt aagcgggact ctggggttcg aaatgaccga 4021 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 4081 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 4141 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat 4201 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg 4261 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc 4321 gagacgccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag 4381 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc 4441 agctcgagta tgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg 4501 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt 4561 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac //