LOCUS Construct_11 4600 bp DNA circular UNA 04-MAY-2014 DEFINITION CMVp-mKate2_EX1-[gRNA1-28]HSV1-mKate2_EX2-pA. ACCESSION urn.local...1397744376719.307 VERSION urn.local...1397744376719.307 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(463..1323) /created_by="User" /label="AmpR" rep_origin 1455..1910 /created_by="User" /label="phage f1 origin of replication" promoter 2039..2627 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature order(2592..2598,2592..2598) /created_by="User" /label="TATA box" misc_signal 2634..2639 /created_by="User" /label="KOZAK" gene 2643..3524 /Original_Bases /modified_by="User" /label="mKate2" misc_feature 2910..2915 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /label="Donor Site" gRNA 2916..3019 /created_by="User" /modified_by="User" /label="gRNA1" misc_feature 3020..3047 /created_by="User" /label="28nt Csy4 rpt" misc_signal 3039..3040 /created_by="User" /label="Csy4 cleaves b/w G&C" misc_feature 3048..3054 /created_by="User" /label="Adeno_branch_point" misc_feature 3055..3059 /created_by="User" /label="XhoI_part" misc_feature 3060..3075 /created_by="User" /modified_by="User" /label="Poly_pirimidine tract" misc_feature 3076..3080 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /label="Acceptor\Site" polyA_signal 3531..3983 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 61 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 121 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 301 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 361 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 421 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 481 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 541 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 601 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 661 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 721 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 781 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 841 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 901 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 961 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 1021 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 1081 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 1141 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 1201 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 1261 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 1321 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 1381 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 1441 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 1501 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 1561 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 1621 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 1681 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 1741 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 1801 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 1861 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc 1921 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 1981 gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg gaggtaccta 2041 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 2101 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 2161 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 2221 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 2281 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 2341 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 2401 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat 2461 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 2521 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac 2581 ggtgggaggt ctatataagc agagctggtt tatgaaccgt cagatccgag ctcgccacca 2641 tggtgtctaa gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg 2701 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg 2761 gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca 2821 tcctggctac cagcttcatg tacggcagca aaaccttcat caaccacacc cagggcatcc 2881 ccgacttctt taagcagtcc ttccctgagg taagttagtc gcgtgtagcg aagcagtttt 2941 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac 3001 cgagtcggtg ctttttttcg ttcactgccg tataggcagc taagaaagag ggagtcgagt 3061 cttctttttt tttttcacag ggcttcacat gggagagagt caccacatac gaagacgggg 3121 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca 3181 agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct 3241 gggaggcctc caccgagatg ctgtaccccg ctgacggcgg cctggaaggc agaagcgaca 3301 tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttgaagacc acatacagat 3361 ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac agaagactgg 3421 aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca 3481 gatactgcga cctccctagc aaactggggc acaaacttaa ttgacccggg taagcgggac 3541 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 3601 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 3661 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac 3721 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 3781 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 3841 gcactctgtc gataccccac cgagacgcca ttggggccaa tacgcccgcg tttcttcctt 3901 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 3961 ggcaggccct gccatagcct cagctcgagt atgtcgaccg atgcccttga gagccttcaa 4021 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt 4081 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca 4141 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 4201 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 4261 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 4321 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 4381 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4441 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat 4501 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 4561 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg //