LOCUS Construct_12 4392 bp DNA circular UNA 05-MAY-2014 DEFINITION CMVp-mKate2_EX1-[gRNA2]consensus-mKate2_EX2-pA. ACCESSION urn.local...1399183024854.82 VERSION urn.local...1399183024854.82 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(206..1066) /created_by="User" /label="AmpR" rep_origin 1198..1653 /created_by="User" /label="phage f1 origin of replication" promoter 1782..2370 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature 2335..2341 /created_by="User" /label="TATA box" misc_signal 2377..2382 /created_by="User" /label="KOZAK" gene 2386..3235 /Original_Bases /modified_by="User" /label="mKate2" misc_feature 2653..>2655 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /label="Donor Site" gRNA 2661..2757 /created_by="User" /modified_by="User" /label="gRNA2" misc_feature 2758..2765 /created_by="User" /label="branch point" misc_feature 2766..2770 /created_by="User" /label="XhoI_part" misc_feature 2771..2786 /created_by="User" /modified_by="User" /label="Poly_pirimidine tract" misc_feature 2787..2791 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /label="Acceptor\Site" polyA_signal 3239..3518 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 61 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 1021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 1081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 1141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 1201 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 1261 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 1321 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 1381 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 1441 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 1501 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 1561 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 1621 cgaattttaa caaaatatta acgtttacaa tttcccattc gccattcagg ctgcgcaact 1681 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat 1741 aagtaagtaa tattaaggta cgggaggtac ttggaggtac ctagttatta atagtaatca 1801 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 1861 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 1921 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 1981 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 2041 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 2101 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg 2161 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc 2221 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt 2281 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 2341 agcagagctg gtttatgaac cgtcagatcc gagctcgcca ccatggtgtc taagggcgaa 2401 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac 2461 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga 2521 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc 2581 atgtacggca gcaaaacctt catcaaccac acccagggca tccccgactt ctttaagcag 2641 tccttccctg aggtaagtcg gagtactgga gtactgtcct gttttagagc tagaaatagc 2701 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcttac 2761 taacttcgag tcttcttttt ttttttcaca gggcttcaca tgggagagag tcaccacata 2821 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat 2881 ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa 2941 aacactcggc tgggaggcct ccaccgagat gctgtacccc gctgacggcg gcctggaagg 3001 cagaagcgac atggccctga agctcgtggg cgggggccac ctgatctgca acttgaagac 3061 cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga 3121 cagaagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt 3181 ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta attgataacc 3241 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac 3301 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt 3361 tcggtcccag ggctggcact ctgtcgatac cccaccgaga cgccattggg gccaatacgc 3421 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc 3481 agccaacgtc ggggcggcag gccctgccat agcctcagct cgagtatgtc gaccgatgcc 3541 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc 3601 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt 3661 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 3721 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 3781 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 3841 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 3901 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 3961 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 4021 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 4081 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 4141 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 4201 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 4261 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 4321 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 4381 tttttgtttg ca //