LOCUS Construct_13 4768 bp DNA circular UNA 24-APR-2014 DEFINITION CMVp-mKate2-Triplex-HHRibo-gRNA1-HDVRibo-pA. ACCESSION urn.local...1397729720410.85 VERSION urn.local...1397729720410.85 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(589..1449) /created_by="User" /label="AmpR" rep_origin 1581..2036 /created_by="User" /label="phage f1 origin of replication" promoter 2165..2754 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature 2718..2724 /created_by="User" /label="TATA box" misc_signal 2761..2766 /created_by="User" /label="KOZAK" gene 2770..3477 /Original_Bases /modified_by="User" /label="mKate2" CDS 3490..3599 /created_by="User" /label="Malat1_3'" misc_RNA 3600..3642 /created_by="User" /label="5' Self cleaving Ribozyme" misc_feature 3600..3605 /created_by="User" /label="Complementary to the first 6 gRNA bps" gRNA 3643..3742 /created_by="User" /modified_by="User" /label="gRNA1" misc_RNA 3743..3810 /created_by="User" /modified_by="User" /label="3' Self cleaving Ribozyme" polyA_signal 3828..4277 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt 61 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 241 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 1021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 1081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 1141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 1201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 1261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 1321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 1381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 1441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 1501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 1561 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 1621 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 1681 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 1741 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 1801 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 1861 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 1921 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 1981 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca 2041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 2101 acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg tacttggagg 2161 tacctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 2221 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 2281 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 2341 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 2401 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 2461 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 2521 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 2581 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 2641 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 2701 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccaagctc 2761 gccaccatgg tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac 2821 atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc 2881 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc 2941 ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa ccacacccag 3001 ggcatccccg acttctttaa gcagtccttc cctgagggct tcacatggga gagagtcacc 3061 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc 3121 ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag 3181 aagaaaacac tcggctggga ggcctccacc gagatgctgt accccgctga cggcggcctg 3241 gaaggcagaa gcgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttg 3301 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat 3361 gtggacagaa gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac 3421 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaattga 3481 taaaccggtg attcgtcagt agggttgtaa aggtttttct tttcctgaga aaacaacctt 3541 ttgttttctc aggttttgct ttttggcctt tccctagctt taaaaaaaaa aaagcaaaac 3601 gactactgat gagtccgtga ggacgaaacg agtaagctcg tctagtcgcg tgtagcgaag 3661 cagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa 3721 gtggcaccga gtcggtgctt ttggccggca tggtcccagc ctcctcgctg gcgccggctg 3781 ggcaacatgc ttcggcatgg cgaatgggac cccgggatgc tagctaagcg ggactctggg 3841 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 3901 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 3961 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac 4021 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 4081 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 4141 tgtcgatacc ccaccgagac gccattgggg ccaatacgcc cgcgtttctt ccttttcccc 4201 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 4261 ccctgccata gcctcagctc gagtatgtcg accgatgccc ttgagagcct tcaacccagt 4321 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt 4381 tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact 4441 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 4501 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 4561 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 4621 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 4681 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 4741 ttaccggata cctgtccgcc tttctccc //