LOCUS Construct_14 4658 bp DNA circular UNA 04-MAY-2014 DEFINITION CMVp-mKate2-HHRibo-gRNA1-HDVRibo-pA. ACCESSION urn.local...1397729720428.86 VERSION urn.local...1397729720428.86 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(479..1339) /created_by="User" /label="AmpR" rep_origin 1471..1926 /created_by="User" /label="phage f1 origin of replication" promoter 2055..2644 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature 2608..2614 /created_by="User" /label="TATA box" misc_feature 2645 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" misc_signal 2651..2656 /created_by="User" /label="KOZAK" gene 2660..3367 /Original_Bases /modified_by="User" /label="mKate2" misc_RNA 3380..3422 /created_by="User" /label="5' Self cleaving Ribozyme" misc_feature 3380..3385 /created_by="User" /label="Complementary to the first 6 gRNA bps" gRNA 3423..3442 /created_by="User" /modified_by="User" /label="gRNA1" misc_feature 3443..3522 /created_by="User" /modified_by="User" /label="gRNA scaffold" misc_RNA 3523..3590 /created_by="User" /modified_by="User" /label="3' Self cleaving Ribozyme" polyA_signal 3608..4057 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 61 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 121 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 181 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 241 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 301 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 361 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 421 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 481 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 541 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 601 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 661 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 721 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 781 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 841 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 901 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 961 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 1021 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 1081 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 1141 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 1201 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 1261 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 1321 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 1381 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 1441 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg 1501 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 1561 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 1621 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 1681 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 1741 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 1801 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 1861 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta 1921 caatttccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 1981 cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg 2041 tacttggagg tacctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 2101 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 2161 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 2221 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 2281 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 2341 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 2401 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg 2461 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg 2521 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg 2581 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag 2641 atccaagctc gccaccatgg tgtctaaggg cgaagagctg attaaggaga acatgcacat 2701 gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga 2761 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct 2821 ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa 2881 ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct tcacatggga 2941 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca 3001 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc 3061 tgtgatgcag aagaaaacac tcggctggga ggcctccacc gagatgctgt accccgctga 3121 cggcggcctg gaaggcagaa gcgacatggc cctgaagctc gtgggcgggg gccacctgat 3181 ctgcaacttg aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg 3241 cgtctactat gtggacagaa gactggaaag aatcaaggag gccgacaaag agacctacgt 3301 cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa 3361 acttaattga taaaccggtc gactactgat gagtccgtga ggacgaaacg agtaagctcg 3421 tctagtcgcg tgtagcgaag cagttttaga gctagaaata gcaagttaaa ataaggctag 3481 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttggccggca tggtcccagc 3541 ctcctcgctg gcgccggctg ggcaacatgc ttcggcatgg cgaatgggac cccgggatgc 3601 tagctaagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 3661 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 3721 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 3781 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 3841 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 3901 cggtcccagg gctggcactc tgtcgatacc ccaccgagac gccattgggg ccaatacgcc 3961 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 4021 gccaacgtcg gggcggcagg ccctgccata gcctcagctc gagtatgtcg accgatgccc 4081 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 4141 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc 4201 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 4261 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 4321 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 4381 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 4441 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 4501 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 4561 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 4621 gctgggctgt gtgcacgaac cccccgttca gcccgacc //