LOCUS Construct_16 5003 bp DNA circular UNA 08-MAY-2014 DEFINITION CMVp-mKate2_EX1-[28-gRNA1-28]HSV1-mKate2_EX2-Triplex-28-gRNA2-28-pA ACCESSION urn.local...1397744417299.330 VERSION urn.local...1397744417299.330 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(302..1162) /created_by="User" /label="AmpR" rep_origin 1294..1749 /created_by="User" /label="phage f1 origin of replication" promoter 1878..2466 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature order(2431..2437,2431..2437) /created_by="User" /label="TATA box" misc_signal 2473..2478 /created_by="User" /label="KOZAK" gene 2482..3391 /Original_Bases /modified_by="User" /label="mKate2" misc_feature 2749..2754 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /label="Donor Site" misc_feature 2755..2782 /created_by="User" /label="28nt Csy4 rpt" misc_signal 2774..2775 /created_by="User" /label="Csy4 cleaves b/w G&C" gRNA 2783..2886 /created_by="User" /modified_by="User" /label="gRNA1" misc_feature 2887..2914 /created_by="User" /label="28nt Csy4 rpt" misc_signal 2906..2907 /created_by="User" /label="Csy4 cleaves b/w G&C" misc_feature 2915..2921 /created_by="User" /label="Adeno_branch_point" misc_feature 2922..2926 /created_by="User" /label="XhoI_part" misc_feature 2927..2942 /created_by="User" /modified_by="User" /label="Poly_pirimidine tract" misc_feature 2943..2947 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /label="Acceptor\Site" CDS 3398..3506 /created_by="User" /label="MALAT1_3' triplex" misc_feature 3507..3556 /created_by="User" /label="50bp_rnd1" misc_signal 3557..3584 /created_by="User" /label="28nt" gRNA 3585..3688 /created_by="User" /modified_by="User" /label="gRNA2" misc_signal 3689..3716 /created_by="User" /modified_by="User" /label="28nt" misc_feature 3717..3766 /created_by="User" /label="50bp_rnd2" polyA_signal 3773..4225 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 61 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 121 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 181 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 241 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 301 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 361 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 421 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 481 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 541 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 601 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 661 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 721 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 781 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 841 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 901 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 961 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 1021 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 1081 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 1141 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 1201 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 1261 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg 1321 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 1381 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 1441 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 1501 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 1561 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 1621 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 1681 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt 1741 ttacaatttc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 1801 cctcttcgct attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg 1861 aggtacttgg aggtacctag ttattaatag taatcaatta cggggtcatt agttcatagc 1921 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt atgaaccgtc 2461 agatccgagc tcgccaccat ggtgtctaag ggcgaagagc tgattaagga gaacatgcac 2521 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc 2581 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct 2641 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa aaccttcatc 2701 aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggt aagtgttcac 2761 tgccgtatag gcagctaaga aatagtcgcg tgtagcgaag cagttttaga gctagaaata 2821 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt 2881 tttttcgttc actgccgtat aggcagctaa gaaagaggga gtcgagtctt cttttttttt 2941 ttcacagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc 3001 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt 3061 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac 3121 cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg ccctgaagct 3181 cgtgggcggg ggccacctga tctgcaactt gaagaccaca tacagatcca agaaacccgc 3241 taagaacctc aagatgcccg gcgtctacta tgtggacaga agactggaaa gaatcaagga 3301 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct 3361 ccctagcaaa ctggggcaca aacttaattg aaccggtgat tcgtcagtag ggttgtaaag 3421 gtttttcttt tcctgagaaa acaacctttt gttttctcag gttttgcttt ttggcctttc 3481 cctagcttta aaaaaaaaaa gcaaaactca ccgaggcagt tccataggat ggcaagatcc 3541 tggtatcggt ctgcgagttc actgccgtat aggcagctaa gaaagtaagt cggagtactg 3601 tcctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa 3661 aagtggcacc gagtcggtgc tttttttcgt tcactgccgt ataggcagct aagaaacaaa 3721 caggaatcga atgcaaccgg cgcaggaaca ttgccagcgc atcaaccccg ggtaagcggg 3781 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 3841 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 3901 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta 3961 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga 4021 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc 4081 tggcactctg tcgatacccc accgagacgc cattggggcc aatacgcccg cgtttcttcc 4141 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg 4201 gcggcaggcc ctgccatagc ctcagctcga gtatgtcgac cgatgccctt gagagccttc 4261 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact 4321 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct 4381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 4441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 4501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 4561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 4621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 4681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 4741 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 4801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 4861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 4921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 4981 tagaaggaca gtatttggta tct //