LOCUS Construct_17 5029 bp DNA circular UNA 09-MAY-2014 DEFINITION CMVp-mKate2-Triplex-28-gRNA1-28-gRNA2-28-pA. ACCESSION urn.local...1397951064932.698 VERSION urn.local...1397951064932.698 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(167..1027) /created_by="User" /label="AmpR" rep_origin 1159..1614 /created_by="User" /label="phage f1 origin of replication" promoter 1743..2332 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature 2296..2302 /created_by="User" /label="TATA box" misc_signal 2339..2344 /created_by="User" /label="KOZAK" gene 2348..3055 /Original_Bases /modified_by="User" /label="mKate2" CDS 3068..3177 /created_by="User" /label="Malat1_3'" misc_feature 3178..3227 /created_by="User" /label="50pb_rnd1" misc_feature 3228..3255 /created_by="User" /label="28nt_Csy4_rpt" gRNA 3256..3275 /created_by="User" /modified_by="User" /label="gRNA1" misc_feature 3276..3359 /created_by="User" /label="gRNA scaffold" misc_feature 3360..3387 /created_by="User" /label="28nt_Csy4_rpt" misc_feature 3388..3437 /created_by="User" /label="50bp_rnd2" protein_bind 3470..3497 /created_by="User" /label="28nt" gRNA 3498..3517 /created_by="User" /modified_by="User" /label="gRNA2" misc_feature 3518..3601 /created_by="User" /label="gRNA_sc" protein_bind 3602..3629 /created_by="User" /label="28nt" polyA_signal 3667..4116 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 61 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 1021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 1081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 1141 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 1201 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 1261 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 1321 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 1381 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 1441 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 1501 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 1561 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt 1621 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 1681 gccagcccaa gctaccatga taagtaagta atattaaggt acgggaggta cttggaggta 1741 cctagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc 1801 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 1861 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 1921 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 1981 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 2041 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 2101 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg 2161 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 2221 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 2281 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccaagctcgc 2341 caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat 2401 ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta 2461 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt 2521 cgacatcctg gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg 2581 catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac 2641 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct 2701 catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa 2761 gaaaacactc ggctgggagg cctccaccga gatgctgtac cccgctgacg gcggcctgga 2821 aggcagaagc gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttgaa 2881 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt 2941 ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga 3001 ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac ttaattgata 3061 aaccggtgat tcgtcagtag ggttgtaaag gtttttcttt tcctgagaaa acaacctttt 3121 gttttctcag gttttgcttt ttggcctttc cctagcttta aaaaaaaaaa agcaaaactc 3181 accgaggcag ttccatagga tggcaagatc ctggtattgg tctgcgagtt cactgccgta 3241 taggcagcta agaaatagtc gcgtgtagcg aagcagtttt agagctagaa atagcaagtt 3301 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttcg 3361 ttcactgccg tataggcagc taagaaacaa acaggaatcg aatgcaaccg gcgcaggaac 3421 actgccagcg catcaacccc gggggcaaga tcctggtatc ggtctgcgag ttcactgccg 3481 tataggcagc taagaaagta agtcggagta ctgtcctgtt ttagagctag aaatagcaag 3541 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt 3601 cgttcactgc cgtataggca gctaagaaac aaacaggaat cgaatgcaac cggcgatgct 3661 agctaagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 3721 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 3781 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta 3841 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg 3901 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc 3961 ggtcccaggg ctggcactct gtcgataccc caccgagacg ccattggggc caatacgccc 4021 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag 4081 ccaacgtcgg ggcggcaggc cctgccatag cctcagctcg agtatgtcga ccgatgccct 4141 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg 4201 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc 4261 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 4321 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 4381 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 4441 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 4501 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 4561 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 4621 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 4681 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 4741 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 4801 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 4861 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 4921 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 4981 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaag //