LOCUS Construct_19 4774 bp DNA circular UNA 08-MAY-2014 DEFINITION CMVp-mKate2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28. ACCESSION urn.local...1397951064950.699 VERSION urn.local...1397951064950.699 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(202..1062) /created_by="User" /label="AmpR" rep_origin 1194..1649 /created_by="User" /label="phage f1 origin of replication" promoter 1778..2367 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature 2331..2337 /created_by="User" /label="TATA box" misc_signal 2374..2379 /created_by="User" /label="KOZAK" gene 2383..3090 /Original_Bases /modified_by="User" /label="mKate2" CDS 3103..3212 /created_by="User" /label="Malat1_3'" misc_feature 3213..3262 /created_by="User" /label="50pb_rnd1" misc_feature 3263..3290 /created_by="User" /label="28nt_Csy4_rpt" gRNA 3297..3400 /created_by="User" /modified_by="User" /label="gRNA3" misc_feature 3401..3428 /created_by="User" /label="28nt_Csy4_rpt" gRNA 3433..3536 /created_by="User" /modified_by="User" /label="gRNA4" misc_feature 3537..3564 /created_by="User" /label="28nt_Csy4_rpt" gRNA 3569..3672 /created_by="User" /modified_by="User" /label="gRNA5" misc_feature 3673..3700 /created_by="User" /label="28nt_Csy4_rpt" gRNA 3705..3808 /created_by="User" /modified_by="User" /label="gRNA6" misc_feature 3809..3836 /created_by="User" /label="28nt_Csy4_rpt" misc_feature 3843..3902 /created_by="User" /label="HSV-pA residual" ORIGIN 1 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 61 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 241 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 301 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 361 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 421 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 481 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 541 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 601 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 661 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 721 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 781 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 841 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 901 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 961 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 1021 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 1081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 1141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc 1201 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 1261 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 1321 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 1381 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 1441 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 1501 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 1561 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 1621 ttttaacaaa atattaacgt ttacaatttc ccattcgcca ttcaggctgc gcaactgttg 1681 ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt 1741 aagtaatatt aaggtacggg aggtacttgg aggtacctag ttattaatag taatcaatta 1801 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 1861 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 1921 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 1981 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 2041 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 2101 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2161 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2221 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2281 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2341 gagctggttt agtgaaccgt cagatccaag ctcgccacca tggtgtctaa gggcgaagag 2401 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac 2461 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc 2521 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg 2581 tacggcagca aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc 2641 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc 2701 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg 2761 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc 2821 accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaagcgacat ggccctgaag 2881 ctcgtgggcg ggggccacct gatctgcaac ttgaagacca catacagatc caagaaaccc 2941 gctaagaacc tcaagatgcc cggcgtctac tatgtggaca gaagactgga aagaatcaag 3001 gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac 3061 ctccctagca aactggggca caaacttaat tgataaaccg gtgattcgtc agtagggttg 3121 taaaggtttt tcttttcctg agaaaacaac cttttgtttt ctcaggtttt gctttttggc 3181 ctttccctag ctttaaaaaa aaaaaagcaa aactcaccga ggcagttcca taggatggca 3241 agatcctggt atcggtctgc gagttcactg ccgtataggc agctaagaaa gctagcgtgt 3301 actctctgag gtgctcgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt 3361 atcaacttga aaaagtggca ccgagtcggt gctttttttc gttcactgcc gtataggcag 3421 ctaagaaaag gtgacgcaga taagaaccag ttgttttaga gctagaaata gcaagttaaa 3481 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttcgttc 3541 actgccgtat aggcagctaa gaaacagggc atcaagtcag ccatcagcgt tttagagcta 3601 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg 3661 gtgctttttt tcgttcactg ccgtataggc agctaagaaa agtcggagtc accctcctgg 3721 aaacgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa 3781 aagtggcacc gagtcggtgc tttttttcgt tcactgccgt ataggcagct aagaaacccg 3841 ggtaagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 3901 agtatgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg 3961 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca 4021 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4081 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4381 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 4441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4621 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 4681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4741 ccgctggtag cggtggtttt tttgtttgca agca //