LOCUS Construct_2 4199 bp DNA circular UNA 23-APR-2014 DEFINITION PGK1p-Csy4-pA . ACCESSION urn.local...1397728251510.26 VERSION urn.local...1397728251510.26 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers rep_origin 72..527 /created_by="User" /label="phage f1 origin of replication" promoter 656..1176 /created_by="User" /label="pmPGK1" misc_signal 1183..1188 /created_by="User" /label="KOZAK" misc_signal 1189..1218 /created_by="User" /label="6x His tag" sig_peptide 1219..1239 /created_by="User" /label="TEV_recognition_peptide" misc_signal 1236..1237 /created_by="User" /label="TEV cleaves b/w Q and S" CDS <1252..1809 /created_by="User" /modified_by="User" /label="Csy4" misc_feature 1843..1965 /created_by="User" /label="PEST" polyA_signal 1976..2197 /created_by="User" /modified_by="User" /label="SV40 polyA" gene complement(3279..4139) /created_by="User" /label="AmpR" ORIGIN 1 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 61 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 121 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 181 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 241 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 301 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 361 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 421 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 481 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt 541 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc 601 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag gtacctaccg 661 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 721 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 781 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 841 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 901 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 961 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 1021 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 1081 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 1141 ctcatctccg ggcctttcga cctgcatccc aagcttacgc gtgccaccat gaaatcttct 1201 caccatcacc atcaccatga aaacctgtac ttccaatcca atgcagctag cgaccactat 1261 ctggacatca gactgaggcc cgatcctgag ttccctcccg cccagctgat gagcgtgctg 1321 tttggcaagc tgcatcaggc tctggtcgcc caaggcggag acagaatcgg cgtgtccttc 1381 cccgacctgg acgagtcccg gagtcgcctg ggcgagcggc tgagaatcca cgccagcgca 1441 gacgatctgc gcgccctgct ggcccggcct tggctggagg gcctgcggga tcatctgcag 1501 tttggcgagc ccgccgtggt gccacaccca acaccctacc gccaggtgag ccgcgtgcag 1561 gccaagtcaa atcccgagag actgcggcgg aggctgatga ggcgacatga tctgagcgag 1621 gaggaggcca gaaagagaat ccccgacaca gtggccagag ccctggatct gccatttgtg 1681 accctgcgga gccagagcac tggccagcat ttcagactgt tcatcagaca cgggcccctg 1741 caggtgacag ccgaggaggg cggatttaca tgctatggcc tgtctaaagg cggcttcgtg 1801 ccctggttct gatgagagct ctaagcggcc gcataagata tcagccatgg cttcccgccg 1861 gcggtggcgg cgcaggatga tggcacgctg cccatgtctt gtgcccagga gagcgggatg 1921 gaccgtcacc ctgcagcctg tgcttctgct aggatcaatg tgtaggctag tcgagcagac 1981 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc 2041 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa 2101 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag 2161 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgagtatgt cgaccgatgc 2221 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg 2281 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct 2341 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 2401 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 2461 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 2521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 2581 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 2641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 2701 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 2761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 2821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 2881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 2941 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 3001 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 3061 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 3121 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 3181 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 3241 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 3301 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 3361 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 3421 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 3481 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 3541 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 3601 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 3661 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 3721 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 3781 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 3841 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 3901 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 3961 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 4021 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 4081 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 4141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggata //