LOCUS Construct_20 5205 bp DNA circular UNA 05-MAY-2014 DEFINITION CMVp-mKate2_EX1-[miRNA]-mKate2_EX2-Triplex-28-gRNA1-28-pA. ACCESSION urn.local...1397730069398.101 VERSION urn.local...1397730069398.101 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(1027..1887) /created_by="User" /label="AmpR" misc_feature 1130..1205 /created_by="User" /label="rna_lk1" misc_feature 1452..1523 /created_by="User" /label="rna_lk2" rep_origin 2019..2474 /created_by="User" /label="phage f1 origin of replication" promoter 2603..3191 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature order(3156..3162,3156..3162) /created_by="User" /label="TATA box" misc_signal 3198..3203 /created_by="User" /label="KOZAK" gene 3207..3473 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /SECDrawAs="Info only" /SECName="mKate-Exon1" /modified_by="User" /label="mKate2-Exon1" misc_signal 3474..3479 /SECDrawAs="Label" /label="5' donor" misc_feature 3474..3479 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /SECDrawAs="Info only" /SECName="Donor\Site" /label="Donor\Site" misc_signal 3480..3828 /SECDrawAs="Label" /label="miR-FF4" misc_feature 3480..3828 /created_by="User" /SECDrawAs="Info only" /SECName="miR-FF4" /label="miR-FF4" misc_feature 3601..3709 /created_by="User" /SECDrawAs="Info only" /SECName="miR-loop" /label="miR-loop" misc_feature 3625..3646 /created_by="User" /SECDrawAs="Info only" /SECName="FF4 s" /label="FF4 s" misc_feature 3625..3646 /gene="FF4 s" /SECDrawAs="Region" /SECStyleId=1 /label="FF4 s misc" misc_feature 3636 /created_by="User" /SECDrawAs="Info only" /SECName="mut C->T" /label="mut C->T" misc_feature 3636 /gene="mut C->T" /SECDrawAs="Marker" /SECStyleId=1 /label="mut C->T misc" misc_feature complement(3666..3687) /created_by="User" /SECDrawAs="Info only" /SECName="FF4 as" /label="FF4 as" misc_feature complement(3666..3687) /gene="FF4 as" /SECDrawAs="Region" /SECStyleId=1 /label="FF4 as misc" misc_feature 3823..3828 /Recognition_pattern="C^AATTG" /annotation_group="CAATTG" /SECDrawAs="Info only" /SECName="MfeI" /SECDescr="Geneious type: restriction site" /label="MfeI" /note="Geneious type: restriction site" misc_signal 3829..3836 /SECDrawAs="Label" /label="BranchPoint" misc_feature 3829..3836 /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="green" /vntifkey=21 /SECDrawAs="Info only" /SECName="BranchPoint" /label="BranchPoint" misc_feature 3837..3841 /created_by="User" /SECDrawAs="Info only" /SECName="XhoI part" /label="XhoI part" misc_feature 3842..3857 /ApEinfo_fwdcolor="#ff80c0" /ApEinfo_revcolor="green" /vntifkey=21 /SECDrawAs="Info only" /SECName="PolyPyrTract" /label="PolyPyrTract" misc_feature 3842..3857 /gene="PolyPyrTract" /SECDrawAs="Region" /SECStyleId=1 /label="PolyPyrTract misc" misc_signal 3858..3862 /SECDrawAs="Label" /label="3' Acceptor\Site" misc_feature 3858..3862 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /SECDrawAs="Info only" /SECName="Acceptor\Site" /label="Acceptor\Site" gene 3863..4306 /ApEinfo_label="mKate" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /SECDrawAs="Info only" /SECName="mKate-Exon2" /modified_by="User" /label="mKate2-Exon2" CDS 4316..4425 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Malat1_3'" /label="Malat1_3'" misc_feature 4426..4475 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="50pb_rnd1" /label="50pb_rnd1" misc_feature 4476..4503 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="28nt_Csy4_rpt" /label="28nt_Csy4_rpt" gRNA 4504..4607 /created_by="User" /SECDrawAs="Label" /modified_by="User" /label="gRNA1" misc_feature 4608..4635 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="28nt_Csy4_rpt" /label="28nt_Csy4_rpt" misc_feature 4636..4685 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="50bp_rnd2" /label="50bp_rnd2" polyA_signal 4700..5152 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa 61 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc 121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 241 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 1021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 1081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 1141 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 1201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 1261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 1321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 1381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 1441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 1501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 1561 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 1621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 1681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 1741 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 1801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 1861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 1921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 1981 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt 2041 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 2101 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 2161 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 2221 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 2281 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 2341 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 2401 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 2461 aacgtttaca atttcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 2521 gcgggcctct tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt 2581 acgggaggta cttggaggta cctagttatt aatagtaatc aattacgggg tcattagttc 2641 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 2701 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 2761 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 2821 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 2881 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 2941 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 3001 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 3061 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 3121 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttatgaa 3181 ccgtcagatc cgagctcgcc accatggtgt ctaagggcga agagctgatt aaggagaaca 3241 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg 3301 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg 3361 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct 3421 tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct gaggtaagtg 3481 tgctcgcttc ggcagcacat atactatgtt gaatgaggct tcagtacttt acagaatcgt 3541 tgcctgcaca tcttggaaac acttgctggg attacttctt caggttaacc caacagaagg 3601 ctcgagtgct gttgacagtg agcgccgctt gaagtcttta attaaatagt gaagccacag 3661 atgtatttaa ttaaagactt caagcggtgc ctactgcctc ggagaattca aggggctact 3721 ttaggagcaa ttatcttgtt tactaaaact gaataccttg ctatctcttt gatacatttt 3781 tacaaagctg aattaaaatg gtataaatta aatcactttt ttcaattgta ctaacttcga 3841 gtcttctttt tttttttcac agggcttcac atgggagaga gtcaccacat acgaagacgg 3901 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt 3961 caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg 4021 ctgggaggcc tccaccgaga tgctgtaccc cgctgacggc ggcctggaag gcagaagcga 4081 catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttgaaga ccacatacag 4141 atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg acagaagact 4201 ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc 4261 cagatactgc gacctcccta gcaaactggg gcacaaactt aattgataaa ccggtgattc 4321 gtcagtaggg ttgtaaaggt ttttcttttc ctgagaaaac aaccttttgt tttctcaggt 4381 tttgcttttt ggcctttccc tagctttaaa aaaaaaaaag caaaactcac cgaggcagtt 4441 ccataggatg gcaagatcct ggtattggtc tgcgagttca ctgccgtata ggcagctaag 4501 aaatagtcgc gtgtagcgaa gcagttttag agctagaaat agcaagttaa aataaggcta 4561 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttcgtt cactgccgta 4621 taggcagcta agaaacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca 4681 tcaaccccgg gatgctagct aagcgggact ctggggttcg aaatgaccga ccaagcgacg 4741 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 4801 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 4861 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga 4921 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt 4981 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagacgccat 5041 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa 5101 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc agctcgagta 5161 tgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttc //