LOCUS Construct_21 5309 bp DNA circular UNA 08-MAY-2014 DEFINITION CMVp-mKate2_EX1-[miRNA]-mKate2_EX2-Triplex-28-gRNA1-28-4xmiRNA-BS-p A. ACCESSION urn.local...1397950968683.656 VERSION urn.local...1397950968683.656 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(283..1143) /created_by="User" /label="AmpR" rep_origin 1275..1730 /created_by="User" /label="phage f1 origin of replication" promoter 1859..2447 /Original_Bases /modified_by="User" /label="pmCMV" misc_signal order(2412..2418,2412..2418) /created_by="User" /modified_by="User" /label="TATA box" misc_signal 2454..2459 /created_by="User" /label="KOZAK" gene 2463..2729 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /SECDrawAs="Info only" /SECName="mKate-Exon1" /label="mKate-Exon1" misc_signal 2730..2735 /SECDrawAs="Label" /label="5' donor" misc_feature 2730..2735 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /SECDrawAs="Info only" /SECName="Donor\Site" /modified_by="User" /label="Donor Site" misc_feature 2736..3084 /created_by="User" /SECDrawAs="Info only" /SECName="miR-FF4" /label="miR-FF4" misc_feature 3079..3084 /Recognition_pattern="C^AATTG" /annotation_group="CAATTG" /SECDrawAs="Info only" /SECName="MfeI" /SECDescr="Geneious type: restriction site" /label="MfeI" /note="Geneious type: restriction site" misc_signal 3085..3092 /SECDrawAs="Label" /label="BranchPoint" misc_feature 3085..3092 /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="green" /vntifkey=21 /SECDrawAs="Info only" /SECName="BranchPoint" /label="BranchPoint" misc_feature 3093..3097 /created_by="User" /SECDrawAs="Info only" /SECName="XhoI part" /label="XhoI part" misc_feature 3098..3113 /ApEinfo_fwdcolor="#ff80c0" /ApEinfo_revcolor="green" /vntifkey=21 /SECDrawAs="Info only" /SECName="PolyPyrTract" /label="PolyPyrTract" misc_feature 3098..3113 /gene="PolyPyrTract" /SECDrawAs="Region" /SECStyleId=1 /label="PolyPyrTract misc" misc_signal 3114..3118 /SECDrawAs="Label" /label="3' Acceptor\Site" gene 3119..3562 /ApEinfo_label="mKate" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /SECDrawAs="Info only" /SECName="mKate-Exon2" /label="mKate-Exon2" CDS 3572..3681 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Malat1_3'" /label="Malat1_3'" misc_feature 3732..3759 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="28nt_Csy4_rpt" /label="28nt_Csy4_rpt" gRNA 3760..3863 /created_by="User" /SECDrawAs="Label" /modified_by="User" /label="gRNA1" misc_feature 3864..3891 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="28nt_Csy4_rpt" /label="28nt_Csy4_rpt" CDS 3942..3985 /gene="4xFF4 BS (perfect match)" /SECDrawAs="Gene" /SECStyleId=1 /modified_by="User" /label="2xFF4 BS (perfect match) CDS" CDS 4010..4053 /created_by="User" /label="2xFF4BS(Perfect match)" polyA_signal 4060..4512 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 61 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 361 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 421 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 481 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 541 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 601 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 661 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 721 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 781 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 841 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 901 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 961 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 1021 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 1081 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 1141 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 1201 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 1261 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 1321 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 1381 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 1441 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 1501 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 1561 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 1621 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 1681 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc 1741 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 1801 gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg gaggtaccta 1861 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 1921 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 1981 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 2041 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 2101 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 2161 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 2221 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat 2281 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 2341 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac 2401 ggtgggaggt ctatataagc agagctggtt tatgaaccgt cagatccgag ctcgccacca 2461 tggtgtctaa gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg 2521 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg 2581 gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca 2641 tcctggctac cagcttcatg tacggcagca aaaccttcat caaccacacc cagggcatcc 2701 ccgacttctt taagcagtcc ttccctgagg taagtgtgct cgcttcggca gcacatatac 2761 tatgttgaat gaggcttcag tactttacag aatcgttgcc tgcacatctt ggaaacactt 2821 gctgggatta cttcttcagg ttaacccaac agaaggctcg agtgctgttg acagtgagcg 2881 ccgcttgaag tctttaatta aatagtgaag ccacagatgt atttaattaa agacttcaag 2941 cggtgcctac tgcctcggag aattcaaggg gctactttag gagcaattat cttgtttact 3001 aaaactgaat accttgctat ctctttgata catttttaca aagctgaatt aaaatggtat 3061 aaattaaatc acttttttca attgtactaa cttcgagtct tctttttttt tttcacaggg 3121 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga 3181 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc 3241 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagatgct 3301 gtaccccgct gacggcggcc tggaaggcag aagcgacatg gccctgaagc tcgtgggcgg 3361 gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg ctaagaacct 3421 caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg aggccgacaa 3481 agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa 3541 actggggcac aaacttaatt gataaaccgg tgattcgtca gtagggttgt aaaggttttt 3601 cttttcctga gaaaacaacc ttttgttttc tcaggttttg ctttttggcc tttccctagc 3661 tttaaaaaaa aaaaagcaaa actcaccgag gcagttccat aggatggcaa gatcctggta 3721 ttggtctgcg agttcactgc cgtataggca gctaagaaat agtcgcgtgt agcgaagcag 3781 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg 3841 gcaccgagtc ggtgcttttt ttcgttcact gccgtatagg cagctaagaa acaaacagga 3901 atcgaatgca accggcgcag gaacactgcc agcgcatcaa cccgcttgaa gtctttaatt 3961 aaaccgcttg aagtctttaa ttaaacctct ggccacatcg gttcctgctc cgcttgaagt 4021 ctttaattaa accgcttgaa gtctttaatt aaacccgggt aagcgggact ctggggttcg 4081 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct 4141 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc 4201 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga 4261 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc 4321 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg 4381 ataccccacc gagacgccat tggggccaat acgcccgcgt ttcttccttt tccccacccc 4441 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg 4501 ccatagcctc agctcgagta tgtcgaccga tgcccttgag agccttcaac ccagtcagct 4561 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca 4621 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg 4681 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 4741 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 4801 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 4861 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 4921 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 4981 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt 5041 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 5101 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 5161 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 5221 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 5281 tttggtatct gcgctctgct gaagccagt //