LOCUS Construct_25 4415 bp DNA circular UNA 08-MAY-2014 DEFINITION CMVp-mKate2-Triplex-28-gRNA5-28. ACCESSION urn.local...1397950968722.659 VERSION urn.local...1397950968722.659 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(237..1097) /created_by="User" /label="AmpR" rep_origin 1229..1684 /created_by="User" /label="phage f1 origin of replication" promoter 1813..2402 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature 2366..2372 /created_by="User" /label="TATA box" misc_signal 2409..2414 /created_by="User" /label="KOZAK" gene 2418..3125 /Original_Bases /modified_by="User" /label="mKate2" CDS 3138..3247 /created_by="User" /label="Malat1_3'" misc_feature 3297..3324 /created_by="User" /label="28nt_Csy4_rpt" gRNA 3331..3434 /created_by="User" /modified_by="User" /label="gRNA5" misc_feature 3435..3462 /created_by="User" /label="28nt_Csy4_rpt" misc_feature 3519..3578 /created_by="User" /label="HSV-pA residual" ORIGIN 1 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 61 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 121 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 181 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 241 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 301 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 361 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 421 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 481 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 541 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 601 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 661 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 721 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 781 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 841 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 901 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 961 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 1021 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 1081 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 1141 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 1201 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg 1261 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 1321 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 1381 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 1441 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 1501 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 1561 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 1621 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca 1681 atttcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 1741 tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt acgggaggta 1801 cttggaggta cctagttatt aatagtaatc aattacgggg tcattagttc atagcccata 1861 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1921 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 1981 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 2041 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 2101 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 2161 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 2221 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2281 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2341 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 2401 ccaagctcgc caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga 2461 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag 2521 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc 2581 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc 2641 acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga 2701 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg 2761 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg 2821 tgatgcagaa gaaaacactc ggctgggagg cctccaccga gatgctgtac cccgctgacg 2881 gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc cacctgatct 2941 gcaacttgaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg 3001 tctactatgt ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg 3061 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac 3121 ttaattgata aaccggtgat tcgtcagtag ggttgtaaag gtttttcttt tcctgagaaa 3181 acaacctttt gttttctcag gttttgcttt ttggcctttc cctagcttta aaaaaaaaaa 3241 agcaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg ctgcgagttc 3301 actgccgtat aggcagctaa gaaagctagc gcatcaagtc agccatcagc gttttagagc 3361 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt 3421 cggtgctttt tttcgttcac tgccgtatag gcagctaaga aacaaacagg aatcgaatgc 3481 aaccggcgca ggaacactgc cagcgcatca accccgggta agcgggactc tggggttcga 3541 aatgaccgac caagcgacgc ccaacctgcc atcacgagta tgtcgaccga tgcccttgag 3601 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact 3661 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt 3721 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 3781 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 3841 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 3901 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 3961 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 4021 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 4081 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 4141 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 4201 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 4261 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 4321 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 4381 aaagagttgg tagctcttga tccggcaaac aaacc //