LOCUS Construct_26 4416 bp DNA circular UNA 08-MAY-2014 DEFINITION CMVp-mKate2-Triplex-28-gRNA6-28. ACCESSION urn.local...1397950968749.660 VERSION urn.local...1397950968749.660 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(237..1097) /created_by="User" /label="AmpR" rep_origin 1229..1684 /created_by="User" /label="phage f1 origin of replication" promoter 1813..2402 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature 2366..2372 /created_by="User" /label="TATA box" misc_feature 2403 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" misc_signal 2409..2414 /created_by="User" /label="KOZAK" gene 2418..3125 /Original_Bases /modified_by="User" /label="mKate2" CDS 3138..3247 /created_by="User" /label="Malat1_3'" misc_feature 3298..3325 /created_by="User" /label="28nt_Csy4_rpt" gRNA 3332..3435 /created_by="User" /modified_by="User" /label="gRNA6" misc_feature 3436..3463 /created_by="User" /label="28nt_Csy4_rpt" misc_feature 3520..3579 /created_by="User" /modified_by="User" /label="HSV-pA residual" ORIGIN 1 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 61 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 121 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 181 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 241 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 301 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 361 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 421 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 481 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 541 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 601 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 661 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 721 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 781 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 841 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 901 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 961 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 1021 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 1081 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 1141 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 1201 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg 1261 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 1321 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 1381 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 1441 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 1501 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 1561 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 1621 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca 1681 atttcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 1741 tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt acgggaggta 1801 cttggaggta cctagttatt aatagtaatc aattacgggg tcattagttc atagcccata 1861 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1921 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 1981 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 2041 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 2101 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 2161 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 2221 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2281 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2341 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 2401 ccaagctcgc caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga 2461 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag 2521 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc 2581 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc 2641 acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga 2701 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg 2761 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg 2821 tgatgcagaa gaaaacactc ggctgggagg cctccaccga gatgctgtac cccgctgacg 2881 gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc cacctgatct 2941 gcaacttgaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg 3001 tctactatgt ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg 3061 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac 3121 ttaattgata aaccggtgat tcgtcagtag ggttgtaaag gtttttcttt tcctgagaaa 3181 acaacctttt gttttctcag gttttgcttt ttggcctttc cctagcttta aaaaaaaaaa 3241 agcaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgagtt 3301 cactgccgta taggcagcta agaaagctag cggagtcacc ctcctggaaa cgttttagag 3361 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag 3421 tcggtgcttt ttttcgttca ctgccgtata ggcagctaag aaacaaacag gaatcgaatg 3481 caaccggcgc aggaacactg ccagcgcatc aaccccgggt aagcgggact ctggggttcg 3541 aaatgaccga ccaagcgacg cccaacctgc catcacgagt atgtcgaccg atgcccttga 3601 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 3661 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct 3721 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 3781 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 3841 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 3901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 3961 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 4021 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 4081 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 4141 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 4201 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 4261 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 4321 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 4381 aaaagagttg gtagctcttg atccggcaaa caaacc //