LOCUS Construct_27 4177 bp DNA circular UNA 05-MAY-2014 DEFINITION P2_EYFP-pA. ACCESSION urn.local...1399308156321.1125 VERSION urn.local...1399308156321.1125 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers polyA_signal 1..154 /created_by="User" /label="Upstream PolyAdenylation signal and Transcriptional stop site" misc_feature complement(168..487) /created_by="User" /modified_by="User" /label="8x gRNA2 binding sites" promoter 494..>525 /created_by="User" /label="Ade MLP" misc_signal 501..507 /created_by="User" /modified_by="User" /label="TATA box" motif 508..518 /created_by="User" /modified_by="User" /label="MAZ/Sp1 binding site" promoter <549..555 /created_by="User" /label="Ade MLP" misc_signal 556..561 /created_by="User" /label="KOZAK" gene 562..1263 /Original_Bases="AAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTAT CCGCTGGAAGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCC TGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAGGTGGACATCACTTACGC TGAGTACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAAT ACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGT TGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACG TGAATTGCTCAACAGTATGGGCATTTCGCAGCCTACCGTGGTGTTCGTTTCCAAAAAG GGGTTGCAAAAAATTTTGAACGTGCAAAAAAAGCTCCCAATCATCCAAAAAATTATTA TCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATC TCATCTACCTCCCGGTTTTAATGAATACGATTTTGTGCCAGAGTCCTTCGATAGGGAC AAGACAATTGCACTGATCATGAACTCCTCTGGATCTACTGGTCTGCCTAAAGGTGTCG CTCTGCCTCATAGAACTGCCTGCGTGAGATTCTCGCATGCCAGAGATCCTATTTTTGG CAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTT GGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCGAGTCGTCTTAATGTATA GATTTGAAGAAGAGCTGTTTCTGAGGAGCCTTCAGGATTACAAGATTCAAAGTGCGCT GCTGGTGCCAACCCTATTCTCCTTCTTCGCCAAAAGCACTCTGATTGACAAATACGAT TTATCTAATTTACACGAAATTGCTTCTGGTGGCGCTCCCCTCTCTAAGGAAGTCGGGG AAGCGGTTGCCAAGAGGTTCCATCTGCCAGGTATCAGGCAAGGATATGGGCTCACTGA GACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGT AAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGG GCGTTAATCAAAGAGGCGAACTGTGTGTGAGAGGTCCTATGATTATGTCCGGTTATGT AAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGGA GACATAGCTTACTGGGACGAAGACGAACACTTCTTCATCGTTGACCGCCTGAAGTCTC TGATTAAGTACAAAGGCTATCAGGTGGCTCCCGCTGAATTGGAATCCATCTTGCTCCA ACACCCCAACATCTTCGACGCAGGTGTCGCAGGTCTTCCCGACGATGACGCCGGTGAA CTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCG TGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTT TGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAG ATCCTCATAAAGGCCAAGAAGGGCGGAAAGATCGCCGTGTAATTCTAGAGTCGGGGC" /modified_by="User" /label="EYFP" polyA_signal 1283..1504 /created_by="User" /modified_by="User" /label="SV40 polyA" gene complement(2600..3460) /created_by="User" /label="AmpR" rep_origin 3592..4047 /created_by="User" /label="phage f1 origin of replication" ORIGIN 1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta 61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc 121 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat agctagccca ggacagtact 181 ccgacttact taatattttc aggctagccc aggacagtac tccgacttac ttaatatttt 241 caggctagcc caggacagta ctccgactta cttaatattt tcaggctagc ccaggacagt 301 actccgactt acttaatatt ttcaggctag cccaggacag tactccgact tacttaatat 361 tttcaggcta gcccaggaca gtactccgac ttacttaata ttttcaggct agcccaggac 421 agtactccga ctaacttaat attttcaggc tagcccagga cagtactccg acttactaat 481 attttcagct agcggggggc tataaaaggg ggtgggggcg ttcgtccatg cttcgctaca 541 cgcgactacc atggcgccac catgagcagc ggcgccctgc tgttccacgg caagatcccc 601 tacgtggtgg agatggaggg cgatgtggat ggccacacct tcagcatccg cggtaagggc 661 tacggcgatg ccagcgtggg caaggtggat gcccagttca tctgcaccac cggcgatgtg 721 cccgtgccct ggagcaccct ggtgaccacc ctgacctacg gcgcccagtg cttcgccaag 781 tacggccccg agctgaagga tttctacaag agctgcatgc ccgatggcta cgtgcaggag 841 cgcaccatca ccttcgaggg cgatggcaat ttcaagaccc gcgccgaggt gaccttcgag 901 aatggcagcg tgtacaatcg cgtgaagctg aatggccagg gcttcaagaa ggatggccac 961 gtgctgggca agaatctgga gttcaatttc accccccact gcctgtacat ctggggcgat 1021 caggccaatc acggcctgaa gagcgccttc aagatctgcc acgagatcgc cggcagcaag 1081 ggcgatttca tcgtggccga tcacacccag atgaataccc ccatcggcgg cggccccgtg 1141 cacgtgcccg agtaccacca catgagctac cacgtgaagc tgagcaagga tgtgaccgat 1201 caccgcgata atatgagcct gacggagacc gtgcgcgccg tggattgccg caagacctac 1261 ctgtaaggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc 1321 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 1381 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 1441 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt 1501 ggtaaaatcg ataaggatcc ctcgagtatg tcgaccgatg cccttgagag ccttcaaccc 1561 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt 1621 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg 1681 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 1741 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 1801 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 1861 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 1921 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 1981 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 2041 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 2101 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2161 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 2221 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2281 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 2341 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 2401 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 2461 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 2521 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 2581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 2641 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 2701 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 2761 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 2821 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 2881 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 2941 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3001 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 3061 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 3121 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 3181 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 3241 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3301 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3361 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 3421 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 3481 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 3541 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct 3601 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 3661 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 3721 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 3781 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3841 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3901 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 3961 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 4021 ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg 4081 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa 4141 gtaatattaa ggtacgggag gtacttggag cggccgc //