LOCUS Construct_3 4817 bp DNA circular SYN 04-MAY-2014 DEFINITION CMVp-mKate2-Triplex-28-gRNA1-28-pA. ACCESSION SPM917 (modified) VERSION KEYWORDS . SOURCE Unknown ORGANISM Mammalian Unclassified. REFERENCE 1 (bases 1 to 4817) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software SECNOTES|GenBank 4817 bp DNA circular UNA 10-DEC-2013 ACCESSION urn.local...1386326571393.2216 VERSION urn.local...1386326571393.2216 KEYWORDS:. FEATURES Location/Qualifiers gene complement(1038..1898) /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="AmpR" /label="AmpR" rep_origin 2030..2485 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="phage f1 ori" /label="phage f1 origin of replication" promoter 2614..3203 /Original_Bases /modified_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="pmCMV" /label="pmCMV" misc_feature 3167..3173 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="TATA box" /label="TATA box" misc_signal 3210..3215 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="KOZAK" /label="KOZAK" gene 3219..3926 /Original_Bases /modified_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="mKATE" /modified_by="User" /label="mKate2" CDS 3939..4048 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Malat1_3'" /label="Malat1_3'" misc_feature 4049..4098 /created_by="User" /translation="LTEAVP" /SECDrawAs="Gene" /SECStyleId=1 /SECName="50pb_rnd1" /label="50pb_rnd1" misc_feature 4099..4126 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="28nt_Csy4_rpt" /label="28nt_Csy4_rpt" gRNA 4127..4230 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /modified_by="User" /label="gRNA1" misc_feature 4231..4258 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="28nt_Csy4_rpt" /label="28nt_Csy4_rpt" misc_feature 4259..4308 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="50bp_rnd2" /label="50bp_rnd2" polyA_signal 4326..4775 /gene="HSV1-pA" /SECDrawAs="Gene" /SECStyleId=1 /modified_by="User" /label="HSV1-pA CDS" ORIGIN 1 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 61 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac 121 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 181 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 241 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 901 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 961 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1021 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 1081 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 1141 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 1201 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 1261 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 1321 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 1381 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 1441 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 1501 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 1561 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 1621 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 1681 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 1741 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 1801 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 1861 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 1921 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 1981 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt 2041 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 2101 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 2161 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 2221 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 2281 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 2341 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 2401 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 2461 aacaaaatat taacgtttac aatttcccat tcgccattca ggctgcgcaa ctgttgggaa 2521 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt 2581 aatattaagg tacgggaggt acttggaggt acctagttat taatagtaat caattacggg 2641 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 2701 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 2761 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 2821 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 2881 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 2941 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat 3001 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 3061 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc 3121 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 3181 tggtttagtg aaccgtcaga tccaagctcg ccaccatggt gtctaagggc gaagagctga 3241 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca 3301 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg 3361 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg 3421 gcagcaaaac cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc 3481 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta 3541 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga 3601 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gcctccaccg 3661 agatgctgta ccccgctgac ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg 3721 tgggcggggg ccacctgatc tgcaacttga agaccacata cagatccaag aaacccgcta 3781 agaacctcaa gatgcccggc gtctactatg tggacagaag actggaaaga atcaaggagg 3841 ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc 3901 ctagcaaact ggggcacaaa cttaattgat aaaccggtga ttcgtcagta gggttgtaaa 3961 ggtttttctt ttcctgagaa aacaaccttt tgttttctca ggttttgctt tttggccttt 4021 ccctagcttt aaaaaaaaaa aagcaaaact caccgaggca gttccatagg atggcaagat 4081 cctggtattg gtctgcgagt tcactgccgt ataggcagct aagaaatagt cgcgtgtagc 4141 gaagcagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga 4201 aaaagtggca ccgagtcggt gctttttttc gttcactgcc gtataggcag ctaagaaaca 4261 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaaccc cgggatgcta 4321 gctaagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 4381 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 4441 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag 4501 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc 4561 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg 4621 gtcccagggc tggcactctg tcgatacccc accgagacgc cattggggcc aatacgcccg 4681 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc 4741 caacgtcggg gcggcaggcc ctgccatagc ctcagctcga gtatgtcgac cgatgccctt 4801 gagagccttc aacccag //