LOCUS Construct_4 4628 bp DNA circular UNA 04-MAY-2014 DEFINITION CMVp-mKate2_EX1-[28-gRNA1-28]HSV1-mKate2_EX2-pA. ACCESSION urn.local...1397728518036.38 VERSION urn.local...1397728518036.38 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(243..1103) /created_by="User" /label="AmpR" rep_origin 1235..1690 /created_by="User" /label="phage f1 origin of replication" promoter 1819..2407 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature order(2372..2378,2372..2378) /created_by="User" /label="TATA box" misc_signal 2414..2419 /created_by="User" /label="KOZAK" gene 2423..3332 /Original_Bases /modified_by="User" /label="mKate2" misc_feature 2690..2695 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /label="Donor Site" misc_feature 2696..2723 /created_by="User" /label="28nt Csy4 rpt" misc_signal 2715..2716 /created_by="User" /label="Csy4 cleaves b/w G&C" gRNA 2724..2827 /created_by="User" /modified_by="User" /label="gRNA1" misc_feature 2828..2855 /created_by="User" /label="28nt Csy4 rpt" misc_signal 2847..2848 /created_by="User" /label="Csy4 cleaves b/w G&C" misc_feature 2856..2862 /created_by="User" /label="Adeno_branch_point" misc_feature 2863..2867 /created_by="User" /label="XhoI_part" misc_feature 2868..2883 /created_by="User" /modified_by="User" /label="Poly_pirimidine tract" misc_feature 2884..2888 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /label="Acceptor\Site" polyA_signal 3339..3791 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 61 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 121 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 181 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 241 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 301 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 361 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 421 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 481 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 541 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 601 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 661 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 721 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 781 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 841 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 901 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 961 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 1021 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 1081 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 1141 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 1201 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc 1261 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 1321 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 1381 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 1441 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 1501 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 1561 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 1621 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 1681 tttacaattt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 1741 gcctcttcgc tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg 1801 gaggtacttg gaggtaccta gttattaata gtaatcaatt acggggtcat tagttcatag 1861 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 1921 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 1981 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 2041 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 2101 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 2161 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 2221 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 2281 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 2341 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tatgaaccgt 2401 cagatccgag ctcgccacca tggtgtctaa gggcgaagag ctgattaagg agaacatgca 2461 catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg 2521 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc 2581 tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca aaaccttcat 2641 caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg taagtgttca 2701 ctgccgtata ggcagctaag aaatagtcgc gtgtagcgaa gcagttttag agctagaaat 2761 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct 2821 ttttttcgtt cactgccgta taggcagcta agaaagaggg agtcgagtct tctttttttt 2881 tttcacaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg 2941 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg 3001 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca 3061 ccgagatgct gtaccccgct gacggcggcc tggaaggcag aagcgacatg gccctgaagc 3121 tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg 3181 ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg 3241 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc 3301 tccctagcaa actggggcac aaacttaatt gacccgggta agcgggactc tggggttcga 3361 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 3421 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 3481 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 3541 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 3601 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 3661 taccccaccg agacgccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 3721 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 3781 catagcctca gctcgagtat gtcgaccgat gcccttgaga gccttcaacc cagtcagctc 3841 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 3901 gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc 3961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 4021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 4081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 4141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 4201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 4261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 4321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 4381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 4441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 4501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 4561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 4621 ccggcaaa //