LOCUS Construct_5 4215 bp DNA circular UNA 24-APR-2014 DEFINITION P1-EYFP-pA. ACCESSION urn.local...1397731149382.161 VERSION urn.local...1397731149382.161 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(468..1328) /created_by="User" /label="AmpR" rep_origin 1460..1915 /created_by="User" /label="phage f1 origin of replication" polyA_signal 2046..2199 /created_by="User" /label="Upstream polyadenylation signal and transcriptional pause site" misc_feature 2224..2574 /created_by="User" /modified_by="User" /label="8x gRNA1 binding sites" promoter 2581..2614 /created_by="User" /modified_by="User" /label="minimal Ade MLP" misc_signal 2588..2594 /created_by="User" /modified_by="User" /label="TATA box" motif 2595..2605 /created_by="User" /modified_by="User" /label="MAZ/Sp1 binding site" misc_signal 2643..2648 /created_by="User" /label="KOZAK" gene 2649..3350 /Original_Bases="AAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTAT CCGCTGGAAGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCC TGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAGGTGGACATCACTTACGC TGAGTACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAAT ACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGT TGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACG TGAATTGCTCAACAGTATGGGCATTTCGCAGCCTACCGTGGTGTTCGTTTCCAAAAAG GGGTTGCAAAAAATTTTGAACGTGCAAAAAAAGCTCCCAATCATCCAAAAAATTATTA TCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATC TCATCTACCTCCCGGTTTTAATGAATACGATTTTGTGCCAGAGTCCTTCGATAGGGAC AAGACAATTGCACTGATCATGAACTCCTCTGGATCTACTGGTCTGCCTAAAGGTGTCG CTCTGCCTCATAGAACTGCCTGCGTGAGATTCTCGCATGCCAGAGATCCTATTTTTGG CAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTT GGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCGAGTCGTCTTAATGTATA GATTTGAAGAAGAGCTGTTTCTGAGGAGCCTTCAGGATTACAAGATTCAAAGTGCGCT GCTGGTGCCAACCCTATTCTCCTTCTTCGCCAAAAGCACTCTGATTGACAAATACGAT TTATCTAATTTACACGAAATTGCTTCTGGTGGCGCTCCCCTCTCTAAGGAAGTCGGGG AAGCGGTTGCCAAGAGGTTCCATCTGCCAGGTATCAGGCAAGGATATGGGCTCACTGA GACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGT AAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGG GCGTTAATCAAAGAGGCGAACTGTGTGTGAGAGGTCCTATGATTATGTCCGGTTATGT AAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGGA GACATAGCTTACTGGGACGAAGACGAACACTTCTTCATCGTTGACCGCCTGAAGTCTC TGATTAAGTACAAAGGCTATCAGGTGGCTCCCGCTGAATTGGAATCCATCTTGCTCCA ACACCCCAACATCTTCGACGCAGGTGTCGCAGGTCTTCCCGACGATGACGCCGGTGAA CTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCG TGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTT TGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAG ATCCTCATAAAGGCCAAGAAGGGCGGAAAGATCGCCGTGTAATTCTAGAGTCGGGGC" /modified_by="User" /label="EYFP" polyA_signal 3370..3591 /created_by="User" /modified_by="User" /label="SV40 polyA" ORIGIN 1 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 61 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 121 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 181 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 241 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 301 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 361 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 421 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 481 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 541 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 601 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 661 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 721 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 781 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 841 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 901 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 961 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 1021 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 1081 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 1141 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 1201 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 1261 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 1321 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 1381 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 1441 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 1501 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 1561 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 1621 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 1681 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 1741 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 1801 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 1861 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat 1921 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 1981 cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg 2041 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga 2101 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg 2161 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgatagcta gctgaaatat 2221 taatgtcgcg actattaata ttttcaggct agccatgctt cgctacacgc gactattaat 2281 attttcaggc tagccatgct tcgctacacg cgactattaa tattttcaag ctagccatgc 2341 ttcgctacac gcgactatta atattttcag gctagccatg cttcgctaac gcgactatta 2401 atattttcag gctagtcatg cttcgctaca cgcgactatt aatattttca ggctagccat 2461 gcttcgctac acgcgactat taatattttc aggctagcca tgcttcgcta cacgcgacta 2521 ttaatatttt caggctagcc atgcttcgct acacgcgact attaatattt tcaggctagc 2581 ggggggctat aaaagggggt gggggcgttc gtcctgctat ctagcgtcgc gttgaccatg 2641 gcgccaccat gagcagcggc gccctgctgt tccacggcaa gatcccctac gtggtggaga 2701 tggagggcga tgtggatggc cacaccttca gcatccgcgg taagggctac ggcgatgcca 2761 gcgtgggcaa ggtggatgcc cagttcatct gcaccaccgg cgatgtgccc gtgccctgga 2821 gcaccctggt gaccaccctg acctacggcg cccagtgctt cgccaagtac ggccccgagc 2881 tgaaggattt ctacaagagc tgcatgcccg atggctacgt gcaggagcgc accatcacct 2941 tcgagggcga tggcaatttc aagacccgcg ccgaggtgac cttcgagaat ggcagcgtgt 3001 acaatcgcgt gaagctgaat ggccagggct tcaagaagga tggccacgtg ctgggcaaga 3061 atctggagtt caatttcacc ccccactgcc tgtacatctg gggcgatcag gccaatcacg 3121 gcctgaagag cgccttcaag atctgccacg agatcgccgg cagcaagggc gatttcatcg 3181 tggccgatca cacccagatg aataccccca tcggcggcgg ccccgtgcac gtgcccgagt 3241 accaccacat gagctaccac gtgaagctga gcaaggatgt gaccgatcac cgcgataata 3301 tgagcctgac ggagaccgtg cgcgccgtgg attgccgcaa gacctacctg taaggccggc 3361 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc 3421 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta 3481 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg 3541 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata 3601 aggatcgagt atgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt 3661 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg 3721 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 3781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 3841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 3901 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 3961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 4021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 4081 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 4141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 4201 gaccgctgcg cctta //