LOCUS Construct_7 3493 bp DNA circular SYN 09-MAY-2014 DEFINITION U6p-gRNA1. ACCESSION Plasmid_p158_CCW (modified) VERSION KEYWORDS . SOURCE Unknown ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3493) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software SECNOTES|Vector molecule: pGEM-t-Easy Fragment ends: blunt Fragment size: 3017 Insert molecule: U6p-a2m_gRNA Fragment ends: blunt Fragment size: 476. FEATURES Location/Qualifiers terminator complement(90..95) /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Termination signal" /label="Termination signal" misc_feature complement(96..172) /created_by="User" /SECDrawAs="Region" /SECStyleId=1 /SECName="gRNA scaffold" /label="gRNA scaffold" gRNA complement(173..191) /created_by="User" /SECDrawAs="Region" /SECStyleId=1 /modified_by="User" /label="gRNA1" promoter complement(193..510) /created_by="User" /modified_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="U6 promoter" /label="U6 promoter" misc_feature complement(216..221) /created_by="User" /SECDrawAs="Region" /SECStyleId=1 /SECName="TATA box" /label="TATA box" misc_feature complement(239..257) /created_by="User" /SECDrawAs="Region" /SECStyleId=1 /SECName="proximal element" /label="proximal element" misc_feature complement(406..413) /created_by="User" /SECDrawAs="Region" /SECStyleId=1 /SECName="Enhancer" /label="Enhancer" ORIGIN 1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat 61 tcaactttgt acaagaaagc tgggtctaga aaaaaagcac cgactcggtg ccactttttc 121 aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa actgcttcgc 181 tacacgcgac tcggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata 241 ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa 301 ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac 361 agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca aatatgaagg 421 aatcatggga aataggccct cttcctgccc gaccttggta ccggatccag tcgactgaat 481 tggttccttt aaagcctgct tttttgtaca aggtggcact tttcggggaa atgtgcgaat 541 cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg 601 atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag 661 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 721 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 781 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 841 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 901 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 961 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 1021 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 1081 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 1141 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 1201 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 1261 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 1321 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 1381 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 1441 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 1501 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 1561 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 1621 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 1681 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 1741 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1801 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 1861 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 1921 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 1981 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 2041 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 2101 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 2161 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 2221 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 2281 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 2341 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 2401 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 2461 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 2521 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 2581 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 2641 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 2701 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 2761 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat 2821 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg 2881 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 2941 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 3001 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 3061 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 3121 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 3181 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 3241 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 3301 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 3361 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 3421 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat 3481 acgactcact ata //