LOCUS Construct_8 4629 bp DNA circular UNA 13-MAY-2014 DEFINITION CMVp-mKate2_EX1-[28-gRNA1-28]consensus-mKate2_EX2-pA. ACCESSION urn.local...1397950929544.646 VERSION urn.local...1397950929544.646 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(451..1311) /created_by="User" /label="AmpR" rep_origin 1443..1898 /created_by="User" /label="phage f1 origin of replication" promoter 2027..2615 /Original_Bases /modified_by="User" /label="pmCMV" misc_signal 2622..2627 /created_by="User" /label="KOZAK" gene 2631..3541 /Original_Bases /modified_by="User" /label="mKate2" misc_feature 2898..2903 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /label="Donor Site" misc_feature 2904..2931 /created_by="User" /label="28nt Csy4 rpt" misc_signal 2923..2924 /created_by="User" /label="Csy4 cleaves b/w G&C" gRNA 2932..3035 /created_by="User" /modified_by="User" /label="gRNA1" misc_feature 3036..3063 /created_by="User" /label="28nt Csy4 rpt" misc_signal 3055..3056 /created_by="User" /label="Csy4 cleaves b/w G&C" misc_feature 3062 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Replacement" misc_feature 3064..3071 /created_by="User" /label="linear_branching_point" misc_feature 3072..3076 /created_by="User" /label="XhoI_part" misc_feature 3077..3092 /created_by="User" /modified_by="User" /label="Poly_pirimidine tract" misc_feature 3093..3097 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /label="Acceptor\Site" misc_feature 3551..4000 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 61 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 121 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 301 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 781 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 841 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 901 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 961 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 1021 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 1081 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 1141 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 1201 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 1261 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 1321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 1381 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 1441 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 1501 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 1561 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 1621 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 1681 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 1741 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 1801 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 1861 taacgcgaat tttaacaaaa tattaacgtt tacaatttcc cattcgccat tcaggctgcg 1921 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc 1981 atgataagta agtaatatta aggtacggga ggtacttgga ggtacctagt tattaatagt 2041 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 2101 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 2161 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 2221 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 2281 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 2341 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt 2401 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc 2461 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat 2521 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct 2581 atataagcag agctggttta tgaaccgtca gatccgagct cgccaccatg gtgtctaagg 2641 gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca 2701 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca 2761 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca 2821 gcttcatgta cggcagcaaa accttcatca accacaccca gggcatcccc gacttcttta 2881 agcagtcctt ccctgaggta agtgttcact gccgtatagg cagctaagaa atagtcgcgt 2941 gtagcgaagc agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa 3001 cttgaaaaag tggcaccgag tcggtgcttt ttttcgttca ctgccgtata ggcagctaag 3061 agatactaac ttcgagtctt cttttttttt ttcacagggc ttcacatggg agagagtcac 3121 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg 3181 cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca 3241 gaagaaaaca ctcggctggg aggcctccac cgagatgctg taccccgctg acggcggcct 3301 ggaaggcaga agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt 3361 gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta 3421 tgtggacaga agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca 3481 cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaattg 3541 acccgggtaa gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 3601 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 3661 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 3721 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg 3781 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg 3841 gttcggtccc agggctggca ctctgtcgat accccaccga gacgccattg gggccaatac 3901 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc 3961 gcagccaacg tcggggcggc aggccctgcc atagcctcag ctcgagtatg tcgaccgatg 4021 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc 4081 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc 4141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 4201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 4261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 4321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 4381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 4441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4501 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4621 ctatcgtct //