LOCUS Construct_9 4962 bp DNA circular UNA 04-MAY-2014 DEFINITION CMVp-mKate2_EX1-[28-gRNA1-28]snoRNA2-mKate2_EX2-pA. ACCESSION urn.local...1397729605286.79 VERSION urn.local...1397729605286.79 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(784..1644) /created_by="User" /label="AmpR" rep_origin 1776..2231 /created_by="User" /label="phage f1 origin of replication" promoter 2360..2948 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature 2913..2919 /created_by="User" /label="TATA box" misc_signal 2955..2960 /created_by="User" /label="KOZAK" gene 2961..3230 /created_by="User" /modified_by="User" /label="mKate2_Ex1" misc_feature 3231..3291 /created_by="User" /label="Genomic Upstream 61bp" misc_feature 3231..3236 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /modified_by="User" /label="Donor Site" misc_signal 3292..3384 /SECDrawAs="Label" /label="5' snoRNA2" misc_feature 3391..3418 /created_by="User" /label="28nt_Csy4_rec_site" gRNA 3419..3522 /created_by="User" /modified_by="User" /label="gRNA1" misc_feature 3529..3556 /created_by="User" /label="28nt_Csy4_rec_site" misc_signal 3563..3656 /created_by="User" /modified_by="User" /label="3' snoRNA2" misc_feature 3663..3723 /created_by="User" /modified_by="User" /label="Genomic downstream 60 bp" misc_feature 3730..3737 /created_by="User" /label="branch point" misc_feature 3738..3742 /created_by="User" /label="XhoI_part" misc_feature 3743..3758 /created_by="User" /modified_by="User" /label="Poly_pirimidine tract" misc_feature 3759..3763 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /vntifkey=21 /label="Acceptor\Site" gene 3764..4204 /created_by="User" /modified_by="User" /label="mKate2_Ex2" polyA_signal 4217..4666 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 61 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 121 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 181 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct 241 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 301 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 361 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 421 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 481 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 541 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 601 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 661 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 721 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 781 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 841 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 901 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 961 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 1021 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 1081 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 1141 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 1201 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 1261 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 1321 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 1381 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 1441 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 1501 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 1561 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 1621 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 1681 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 1741 ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag 1801 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 1861 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 1921 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 1981 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 2041 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 2101 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 2161 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 2221 gtttacaatt tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 2281 ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg 2341 ggaggtactt ggaggtacct agttattaat agtaatcaat tacggggtca ttagttcata 2401 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 2461 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 2521 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 2581 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 2641 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 2701 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 2761 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 2821 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 2881 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttatgaaccg 2941 tcagatccga gctcgccacc atggtgtcta agggcgaaga gctgattaag gagaacatgc 3001 acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg 3061 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc 3121 ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc aaaaccttca 3181 tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccctgag gtaagtgttc 3241 atttctcaaa agaccctaat gttcttcctt tacaggaatg aatactgtgc atggaccaat 3301 gatgacttcc atacatgcat tccttggaaa gctgaacaaa atgagtggga actctgtact 3361 atcatcttag ttgaactgag gtccggatcc gttcactgcc gtataggcag ctaagaaata 3421 gtcgcgtgta gcgaagcagt tttagagcta gaaatagcaa gttaaaataa ggctagtccg 3481 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tcagatctgt tcactgccgt 3541 ataggcagct aagaaatcta gatggatcga tgatgacttc catatataca ttccttggaa 3601 agctgaacaa aatgagtgaa aactctatac cgtcattctc gtcgaactga ggtccaaccg 3661 gtgcacatta ctccaacagg ggctagacag agagggccaa cattgattcg ttgacatggg 3721 tggctgcagt actaacttcg agtcttcttt ttttttttca cagggcttca catgggagag 3781 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga 3841 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt 3901 gatgcagaag aaaacactcg gctgggaggc ctccaccgag atgctgtacc ccgctgacgg 3961 cggcctggaa ggcagaagcg acatggccct gaagctcgtg ggcgggggcc acctgatctg 4021 caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt 4081 ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga 4141 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaact 4201 taattgaccc gggtaagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 4261 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 4321 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 4381 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 4441 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 4501 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacg ccattggggc 4561 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 4621 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcagctcg agtatgtcga 4681 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact 4741 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca 4801 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 4861 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 4921 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cg //