LOCUS SPM1128 4781 bp DNA circular SYN 21-MAY-2014 DEFINITION CMVp-dsRed2-Triplex-28-gRNA1-28-pA. ACCESSION SPM917 (modified) VERSION KEYWORDS . SOURCE Unknown ORGANISM Mammalian Unclassified. REFERENCE 1 (bases 1 to 4817) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software SECNOTES|GenBank 4817 bp DNA circular UNA 10-DEC-2013 ACCESSION urn.local...1386326571393.2216 VERSION urn.local...1386326571393.2216 KEYWORDS:. FEATURES Location/Qualifiers gene complement(1038..1898) /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="AmpR" /label="AmpR" rep_origin 2030..2485 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="phage f1 ori" /label="phage f1 origin of replication" promoter 2614..3203 /Original_Bases /modified_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="pmCMV" /label="pmCMV" misc_feature 3167..3173 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="TATA box" /label="TATA box" misc_signal 3210..3215 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="KOZAK" /label="KOZAK" gene 3216..3890 /created_by="User" /label="dsRed2" CDS 3903..4012 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Malat1_3'" /label="Malat1_3'" misc_feature 4013..4062 /created_by="User" /translation="LTEAVP" /SECDrawAs="Gene" /SECStyleId=1 /SECName="50pb_rnd1" /label="50pb_rnd1" misc_feature 4063..4090 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="28nt_Csy4_rpt" /label="28nt_Csy4_rpt" gRNA 4091..4194 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /modified_by="User" /label="gRNA1" misc_feature 4195..4222 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="28nt_Csy4_rpt" /label="28nt_Csy4_rpt" misc_feature 4223..4272 /created_by="User" /SECDrawAs="Gene" /SECStyleId=1 /SECName="50bp_rnd2" /label="50bp_rnd2" polyA_signal 4290..4739 /gene="HSV1-pA" /SECDrawAs="Gene" /SECStyleId=1 /modified_by="User" /label="HSV1-pA CDS" ORIGIN 1 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 61 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac 121 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 181 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 241 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 901 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 961 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1021 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 1081 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 1141 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 1201 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 1261 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 1321 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 1381 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 1441 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 1501 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 1561 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 1621 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 1681 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 1741 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 1801 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 1861 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 1921 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 1981 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt 2041 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 2101 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 2161 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 2221 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 2281 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 2341 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 2401 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 2461 aacaaaatat taacgtttac aatttcccat tcgccattca ggctgcgcaa ctgttgggaa 2521 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt 2581 aatattaagg tacgggaggt acttggaggt acctagttat taatagtaat caattacggg 2641 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 2701 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 2761 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 2821 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 2881 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 2941 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat 3001 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 3061 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc 3121 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 3181 tggtttagtg aaccgtcaga tccgagctcg ccaccatggc ctcctccgag aacgtcatca 3241 ccgagttcat gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac gagttcgaga 3301 tcgagggcga gggcgagggc cgcccctacg agggccacaa caccgtgaag ctgaaggtga 3361 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct 3421 ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg 3481 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc 3541 aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc ggcgtgaact 3601 tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc tccaccgagc 3661 gcctgtaccc ccgcgacggc gtgctgaagg gcgagaccca caaggccctg aagctgaagg 3721 acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc 3781 tgcccggcta ctactacgtg gacgccaagc tggacatcac ctcccacaac gaggactaca 3841 ccatcgtgga gcagtacgag cgcaccgagg gccgccacca cctgttcctg tagtaaaccg 3901 gtgattcgtc agtagggttg taaaggtttt tcttttcctg agaaaacaac cttttgtttt 3961 ctcaggtttt gctttttggc ctttccctag ctttaaaaaa aaaaaagcaa aactcaccga 4021 ggcagttcca taggatggca agatcctggt attggtctgc gagttcactg ccgtataggc 4081 agctaagaaa tagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat 4141 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttcgttcac 4201 tgccgtatag gcagctaaga aacaaacagg aatcgaatgc aaccggcgca ggaacactgc 4261 cagcgcatca accccgggat gctagctaag cgggactctg gggttcgaaa tgaccgacca 4321 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 4381 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 4441 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc 4501 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc 4561 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag 4621 acgccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc 4681 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagc 4741 tcgagtatgt cgaccgatgc ccttgagagc cttcaaccca g //