LOCUS SPM1129 4732 bp DNA circular UNA 21-MAY-2014 DEFINITION CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA. ACCESSION urn.local...1400693331756.181 VERSION urn.local...1400693331756.181 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers gene complement(589..1449) /created_by="User" /label="AmpR" rep_origin 1581..2036 /created_by="User" /label="phage f1 origin of replication" promoter 2165..2754 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature 2718..2724 /created_by="User" /label="TATA box" misc_signal 2761..2766 /created_by="User" /label="KOZAK" gene 2767..3441 /created_by="User" /label="dsRed2" CDS 3454..3563 /created_by="User" /label="Malat1_3'" misc_RNA 3564..3606 /created_by="User" /label="5' Self cleaving Ribozyme" misc_feature 3564..3569 /created_by="User" /label="Complementary to the first 6 gRNA bps" gRNA 3607..3706 /created_by="User" /modified_by="User" /label="gRNA1" misc_RNA 3707..3774 /created_by="User" /modified_by="User" /label="3' Self cleaving Ribozyme" polyA_signal 3792..4241 /Original_Bases /modified_by="User" /label="HSV polyA" ORIGIN 1 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt 61 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 241 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 1021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 1081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 1141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 1201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 1261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 1321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 1381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 1441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 1501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 1561 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 1621 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 1681 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 1741 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 1801 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 1861 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 1921 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 1981 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca 2041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 2101 acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg tacttggagg 2161 tacctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 2221 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 2281 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 2341 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 2401 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 2461 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 2521 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 2581 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 2641 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 2701 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgagctc 2761 gccaccatgg cctcctccga gaacgtcatc accgagttca tgcgcttcaa ggtgcgcatg 2821 gagggcaccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 2881 gagggccaca acaccgtgaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg 2941 gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac 3001 atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 3061 ttcgaggacg gcggcgtggc gaccgtgacc caggactcct ccctgcagga cggctgcttc 3121 atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt gatgcagaag 3181 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag 3241 ggcgagaccc acaaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag 3301 tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt ggacgccaag 3361 ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga gcgcaccgag 3421 ggccgccacc acctgttcct gtagtaaacc ggtgattcgt cagtagggtt gtaaaggttt 3481 ttcttttcct gagaaaacaa ccttttgttt tctcaggttt tgctttttgg cctttcccta 3541 gctttaaaaa aaaaaaagca aaacgactac tgatgagtcc gtgaggacga aacgagtaag 3601 ctcgtctagt cgcgtgtagc gaagcagttt tagagctaga aatagcaagt taaaataagg 3661 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttggcc ggcatggtcc 3721 cagcctcctc gctggcgccg gctgggcaac atgcttcggc atggcgaatg ggaccccggg 3781 atgctagcta agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 3841 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 3901 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 3961 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat 4021 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg 4081 ggttcggtcc cagggctggc actctgtcga taccccaccg agacgccatt ggggccaata 4141 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct 4201 cgcagccaac gtcggggcgg caggccctgc catagcctca gctcgagtat gtcgaccgat 4261 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 4321 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 4381 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 4441 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 4501 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 4561 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 4621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 4681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc cc //