LOCUS SPM1130 5137 bp DNA circular UNA 21-MAY-2014 DEFINITION CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA. ACCESSION urn.local...1400693331772.182 VERSION urn.local...1400693331772.182 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers primer_bind 558..580 /created_by="primer3" /%GC="69.6" /Tm="64.72" /Hairpin="4.0" /Primer-Dimer="4.0" /Monovalent_Salt_Concentration="50.0" /Divalent_Salt_Concentration="0.0" /DNTP_Concentration="0.0" /Sequence="GGGGTTCCGCGCACATTTCCCCG" /Mismatches=0 /annotation_group="PrimerPlugin Groupcf8ddb68-38ad-460d-b4f5-813b1c005994_urn:local:.:1365 731840203.183" /label="JP313" rep_origin 595..1050 /created_by="User" /label="phage f1 origin of replication" primer_bind 1104..1124 /created_by="primer3" /%GC="57.1" /Tm="56.74" /Hairpin="3.0" /Primer-Dimer="0.0" /Monovalent_Salt_Concentration="50.0" /Divalent_Salt_Concentration="0.0" /DNTP_Concentration="0.0" /Sequence="TCTTCGCTATTACGCCAGCCC" /Mismatches=0 /annotation_group="PrimerPlugin Group98f9b99e-9408-4564-8d05-995754f0fc42_urn:local:.:1365 731840203.183" /label="JP244" promoter 1179..1768 /Original_Bases /modified_by="User" /label="pmCMV" primer_bind 1179..1216 /created_by="primer3" /%GC="28.9" /Tm="55.98" /Hairpin="10.0" /Primer-Dimer="3.0" /Monovalent_Salt_Concentration="50.0" /Divalent_Salt_Concentration="0.0" /DNTP_Concentration="0.0" /Sequence="TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTC" /Mismatches=0 /Extension="AAGTAAGTAATATTAAGGTACGGGAGGTACTTGGAGGTACC" /annotation_group="PrimerPlugin Groupfc7c65d0-d838-4ff7-8eae-763b425b147b_urn:local:.:1365 731840203.183" /label="JP440" misc_feature 1732..1738 /created_by="User" /label="TATA box" misc_feature complement(1742..1768) /created_by="primer3" /%GC="51.9" /Tm="59.19" /Hairpin="7.0" /Primer-Dimer="5.0" /Monovalent_Salt_Concentration="50.0" /Divalent_Salt_Concentration="0.0" /DNTP_Concentration="0.0" /Sequence="GGATCTGACGGTTCACTAAACCAGCTC" /Mismatches=0 /Extension="CTTCCTCTTCTTCTTGGGCATGGTGGCACGCGT" /annotation_group="PrimerPlugin Group7b9097d0-be18-4341-b3d5-50b254a17947_urn:local:.:1365 731840203.183" /label="JP441" /note="Geneious type: primer_bind_reverse" misc_signal 1775..1780 /created_by="User" /label="KOZAK" gene 1781..2455 /created_by="User" /label="dsRed2" CDS 2468..2577 /created_by="User" /label="Malat1_3'" misc_feature 2578..2627 /created_by="User" /label="50pb_rnd1" misc_feature 2628..2655 /created_by="User" /label="28nt_Csy4_rpt" gRNA 2662..2681 /created_by="User" /modified_by="User" /label="IL1RN_CR1" misc_feature 2682..2765 /created_by="User" /label="gRNA scaffold" misc_feature 2766..2793 /created_by="User" /label="28nt_Csy4_rpt" gRNA 2798..2817 /created_by="User" /modified_by="User" /label="IL1RN_CR2" misc_feature 2818..2901 /created_by="User" /label="gRNA scaffold" misc_feature 2902..2929 /created_by="User" /label="28nt_Csy4_rpt" gRNA 2934..2953 /created_by="User" /modified_by="User" /label="IL1RN_CR3" misc_feature 2954..3037 /created_by="User" /label="gRNA scaffold" misc_feature 3038..3065 /created_by="User" /label="28nt_Csy4_rpt" gRNA 3070..3089 /created_by="User" /label="IL1RN_CR4" misc_feature 3090..3173 /created_by="User" /label="gRNA scaffold" misc_feature 3174..3201 /created_by="User" /label="28nt_Csy4_rpt" polyA_signal 3208..3660 /created_by="User" /label="HSV polyA" gene complement(4740..463) /created_by="User" /label="AmpR" ORIGIN 1 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 61 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc 601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 781 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 901 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 961 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 1021 attttaacaa aatattaacg tttacaattt cccattcgcc attcaggctg cgcaactgtt 1081 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta ccatgataag 1141 taagtaatat taaggtacgg gaggtacttg gaggtaccta gttattaata gtaatcaatt 1201 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 1261 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 1321 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 1381 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 1441 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 1501 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 1561 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 1621 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 1681 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 1741 agagctggtt tagtgaaccg tcagatccga gctcgccacc atggcctcct ccgagaacgt 1801 catcaccgag ttcatgcgct tcaaggtgcg catggagggc accgtgaacg gccacgagtt 1861 cgagatcgag ggcgagggcg agggccgccc ctacgagggc cacaacaccg tgaagctgaa 1921 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta 1981 cggctccaag gtgtacgtga agcaccccgc cgacatcccc gactacaaga agctgtcctt 2041 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggcgaccgt 2101 gacccaggac tcctccctgc aggacggctg cttcatctac aaggtgaagt tcatcggcgt 2161 gaacttcccc tccgacggcc ccgtgatgca gaagaagacc atgggctggg aggcctccac 2221 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag acccacaagg ccctgaagct 2281 gaaggacggc ggccactacc tggtggagtt caagtccatc tacatggcca agaagcccgt 2341 gcagctgccc ggctactact acgtggacgc caagctggac atcacctccc acaacgagga 2401 ctacaccatc gtggagcagt acgagcgcac cgagggccgc caccacctgt tcctgtagta 2461 aaccggtgat tcgtcagtag ggttgtaaag gtttttcttt tcctgagaaa acaacctttt 2521 gttttctcag gttttgcttt ttggcctttc cctagcttta aaaaaaaaaa agcaaaactc 2581 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgagtt cactgccgta 2641 taggcagcta agaaagctag cgtgtactct ctgaggtgct cgttttagag ctagaaatag 2701 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt 2761 ttttcgttca ctgccgtata ggcagctaag aaaaggtgac gcagataaga accagttgtt 2821 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc 2881 accgagtcgg tgcttttttt cgttcactgc cgtataggca gctaagaaac agggcatcaa 2941 gtcagccatc agcgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc 3001 aacttgaaaa agtggcaccg agtcggtgct ttttttcgtt cactgccgta taggcagcta 3061 agaaaagtcg gagtcaccct cctggaaacg ttttagagct agaaatagca agttaaaata 3121 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttcgttcact 3181 gccgtatagg cagctaagaa acccgggtaa gcgggactct ggggttcgaa atgaccgacc 3241 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 3301 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 3361 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac 3421 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt 3481 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 3541 gacgccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 3601 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag 3661 ctcgagtatg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg 3721 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag 3781 gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 3841 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 3901 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 3961 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 4021 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 4081 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 4141 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 4201 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 4261 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4321 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4381 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 4441 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 4501 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 4561 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 4621 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 4681 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 4741 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 4801 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 4861 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 4921 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 4981 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 5041 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 5101 agctccggtt cccaacgatc aaggcgagtt acatgat //