Synthetic Biology Group, Professor Timothy Lu
  1. Bousbaine, D, Fisch, LI, London, M, Bhagchandani, P, Rezende de Castro, TB, Mimee, M, Olesen, S, Reis, BS, VanInsberghe, D, Bortolatto, J, Poyet, M, Cheloha, RW, Sidney, J, Ling, J, Gupta, A, Lu, TK, Sette, A, Alm, EJ, Moon, JJ, Victora, GD et al.. A conserved Bacteroidetes antigen induces anti-inflammatory intestinal T lymphocytes. Science. 2022;377 (6606):660-666. doi: 10.1126/science.abg5645. PubMed PMID:35926021 .
  2. Liu, X, Yang, Y, Inda, ME, Lin, S, Wu, J, Kim, Y, Chen, X, Ma, D, Lu, TK, Zhao, X. Magnetic Living Hydrogels for Intestinal Localization, Retention, and Diagnosis. Adv Funct Mater. 2021;31 (27):. doi: 10.1002/adfm.202010918. PubMed PMID:35903441 PubMed Central PMC9328153.
  3. Lai, Y, Hayashi, N, Lu, TK. Engineering the human gut commensal Bacteroides thetaiotaomicron with synthetic biology. Curr Opin Chem Biol. 2022;70 :102178. doi: 10.1016/j.cbpa.2022.102178. PubMed PMID:35759819 .
  4. Chen, CH, Bepler, T, Pepper, K, Fu, D, Lu, TK. Synthetic molecular evolution of antimicrobial peptides. Curr Opin Biotechnol. 2022;75 :102718. doi: 10.1016/j.copbio.2022.102718. PubMed PMID:35395425 .
  5. Guo, T, Ma, D, Lu, TK. Sense-and-Respond Payload Delivery Using a Novel Antigen-Inducible Promoter Improves Suboptimal CAR-T Activation. ACS Synth Biol. 2022;11 (4):1440-1453. doi: 10.1021/acssynbio.1c00236. PubMed PMID:35316028 PubMed Central PMC9016769.
  6. Chen, CH, Pepper, K, Ulmschneider, JP, Ulmschneider, MB, Lu, TK. Predicting Membrane-Active Peptide Dynamics in Fluidic Lipid Membranes. Methods Mol Biol. 2022;2405 :115-136. doi: 10.1007/978-1-0716-1855-4_6. PubMed PMID:35298811 .
  7. Liu, X, Inda, ME, Lai, Y, Lu, TK, Zhao, X. Engineered Living Hydrogels. Adv Mater. 2022;34 (26):e2201326. doi: 10.1002/adma.202201326. PubMed PMID:35243704 PubMed Central PMC9250645.
  8. Wu, X, Chan, C, Springs, SL, Lee, YH, Lu, TK, Yu, H. A warm-start digital CRISPR/Cas-based method for the quantitative detection of nucleic acids. Anal Chim Acta. 2022;1196 :339494. doi: 10.1016/j.aca.2022.339494. PubMed PMID:35151407 .
  9. Lai, Y, Cui, L, Babunovic, GH, Fortune, SM, Doench, JG, Lu, TK. High-Throughput CRISPR Screens To Dissect Macrophage-Shigella Interactions. mBio. 2021;12 (6):e0215821. doi: 10.1128/mBio.02158-21. PubMed PMID:34933448 PubMed Central PMC8689513.
  10. Cable, J, Leonard, JN, Lu, TK, Xie, Z, Chang, MW, Fernández, LÁ, Lora, JM, Kaufman, HL, Quintana, FJ, Geiger, R, F Lesser, C, Lynch, JP, Hava, DL, Cornish, VW, Lee, GK, DiAndreth, B, Fero, M, Srivastava, R, De Coster, T, Roybal, KT et al.. Synthetic biology: at the crossroads of genetic engineering and human therapeutics-a Keystone Symposia report. Ann N Y Acad Sci. 2021;1506 (1):98-117. doi: 10.1111/nyas.14710. PubMed PMID:34786712 .
  11. Cubillos-Ruiz, A, Guo, T, Sokolovska, A, Miller, PF, Collins, JJ, Lu, TK, Lora, JM. Engineering living therapeutics with synthetic biology. Nat Rev Drug Discov. 2021;20 (12):941-960. doi: 10.1038/s41573-021-00285-3. PubMed PMID:34616030 .
  12. He, J, Nissim, L, Soleimany, AP, Binder-Nissim, A, Fleming, HE, Lu, TK, Bhatia, SN. Synthetic Circuit-Driven Expression of Heterologous Enzymes for Disease Detection. ACS Synth Biol. 2021;10 (9):2231-2242. doi: 10.1021/acssynbio.1c00133. PubMed PMID:34464083 .
  13. Farzadfard, F, Gharaei, N, Citorik, RJ, Lu, TK. Efficient retroelement-mediated DNA writing in bacteria. Cell Syst. 2021;12 (9):860-872.e5. doi: 10.1016/j.cels.2021.07.001. PubMed PMID:34358440 .
  14. Cao, J, Novoa, EM, Zhang, Z, Chen, WCW, Liu, D, Choi, GCG, Wong, ASL, Wehrspaun, C, Kellis, M, Lu, TK. High-throughput 5' UTR engineering for enhanced protein production in non-viral gene therapies. Nat Commun. 2021;12 (1):4138. doi: 10.1038/s41467-021-24436-7. PubMed PMID:34230498 PubMed Central PMC8260622.
  15. Gonzalez-Junca, A, Liu, FD, Nagaraja, AS, Mullenix, A, Lee, CT, Gordley, RM, Frimannsson, DO, Maller, O, Garrison, BS, Iyer, D, Benabbas, A, Truong, TA, Quach, A, Tian, M, Martinez, R, Savur, R, Perry-McNamara, A, Nguyen, D, Almudhfar, N, Blanco, C et al.. SENTI-101, a Preparation of Mesenchymal Stromal Cells Engineered to Express IL12 and IL21, Induces Localized and Durable Antitumor Immunity in Preclinical Models of Peritoneal Solid Tumors. Mol Cancer Ther. 2021;20 (9):1508-1520. doi: 10.1158/1535-7163.MCT-21-0030. PubMed PMID:34210826 .
  16. Wu, X, Tay, JK, Goh, CK, Chan, C, Lee, YH, Springs, SL, Wang, Y, Loh, KS, Lu, TK, Yu, H. Digital CRISPR-based method for the rapid detection and absolute quantification of nucleic acids. Biomaterials. 2021;274 :120876. doi: 10.1016/j.biomaterials.2021.120876. PubMed PMID:34034027 .
  17. Kim, T, Weinberg, B, Wong, W, Lu, TK. Scalable recombinase-based gene expression cascades. Nat Commun. 2021;12 (1):2711. doi: 10.1038/s41467-021-22978-4. PubMed PMID:33976199 PubMed Central PMC8113245.
  18. Schubert, MG, Goodman, DB, Wannier, TM, Kaur, D, Farzadfard, F, Lu, TK, Shipman, SL, Church, GM. High-throughput functional variant screens via in vivo production of single-stranded DNA. Proc Natl Acad Sci U S A. 2021;118 (18):. doi: 10.1073/pnas.2018181118. PubMed PMID:33906944 PubMed Central PMC8106316.
  19. Tang, TC, Tham, E, Liu, X, Yehl, K, Rovner, AJ, Yuk, H, de la Fuente-Nunez, C, Isaacs, FJ, Zhao, X, Lu, TK. Hydrogel-based biocontainment of bacteria for continuous sensing and computation. Nat Chem Biol. 2021;17 (6):724-731. doi: 10.1038/s41589-021-00779-6. PubMed PMID:33820990 PubMed Central PMC9269716.
  20. Cleto, S, Haslinger, K, Prather, KLJ, Lu, TK. Natural combinatorial genetics and prolific polyamine production enable siderophore diversification in Serratia plymuthica. BMC Biol. 2021;19 (1):46. doi: 10.1186/s12915-021-00971-z. PubMed PMID:33722216 PubMed Central PMC7962358.
  21. Lu, Timothy K