Synthetic Biology Group, Professor Timothy Lu
  1. Cao, J, Novoa, EM, Zhang, Z, Chen, WCW, Liu, D, Choi, GCG, Wong, ASL, Wehrspaun, C, Kellis, M, Lu, TK. High-throughput 5' UTR engineering for enhanced protein production in non-viral gene therapies. Nat Commun. 2021;12 (1):4138. doi: 10.1038/s41467-021-24436-7. PubMed PMID:34230498 PubMed Central PMC8260622.
  2. Gonzalez-Junca, A, Liu, FD, Nagaraja, AS, Mullenix, A, Lee, CT, Gordley, RM, Frimannsson, DO, Maller, O, Garrison, BS, Iyer, D, Benabbas, A, Truong, TA, Quach, A, Tian, M, Martinez, R, Savur, R, Perry-McNamara, A, Nguyen, D, Almudhfar, N, Blanco, C et al.. SENTI-101, a preparation of mesenchymal stromal cells engineered to express IL-12 and IL-21, induces localized and durable anti-tumor immunity in preclinical models of peritoneal solid tumors. Mol Cancer Ther. 2021; :. doi: 10.1158/1535-7163.MCT-21-0030. PubMed PMID:34210826 .
  3. Wu, X, Tay, JK, Goh, CK, Chan, C, Lee, YH, Springs, SL, Wang, Y, Loh, KS, Lu, TK, Yu, H. Digital CRISPR-based method for the rapid detection and absolute quantification of nucleic acids. Biomaterials. 2021;274 :120876. doi: 10.1016/j.biomaterials.2021.120876. PubMed PMID:34034027 .
  4. Kim, T, Weinberg, B, Wong, W, Lu, TK. Scalable recombinase-based gene expression cascades. Nat Commun. 2021;12 (1):2711. doi: 10.1038/s41467-021-22978-4. PubMed PMID:33976199 PubMed Central PMC8113245.
  5. Schubert, MG, Goodman, DB, Wannier, TM, Kaur, D, Farzadfard, F, Lu, TK, Shipman, SL, Church, GM. High-throughput functional variant screens via in vivo production of single-stranded DNA. Proc Natl Acad Sci U S A. 2021;118 (18):. doi: 10.1073/pnas.2018181118. PubMed PMID:33906944 PubMed Central PMC8106316.
  6. Tang, TC, Tham, E, Liu, X, Yehl, K, Rovner, AJ, Yuk, H, de la Fuente-Nunez, C, Isaacs, FJ, Zhao, X, Lu, TK. Hydrogel-based biocontainment of bacteria for continuous sensing and computation. Nat Chem Biol. 2021;17 (6):724-731. doi: 10.1038/s41589-021-00779-6. PubMed PMID:33820990 .
  7. Cleto, S, Haslinger, K, Prather, KLJ, Lu, TK. Natural combinatorial genetics and prolific polyamine production enable siderophore diversification in Serratia plymuthica. BMC Biol. 2021;19 (1):46. doi: 10.1186/s12915-021-00971-z. PubMed PMID:33722216 PubMed Central PMC7962358.
  8. Torres, MDT, Cao, J, Franco, OL, Lu, TK, de la Fuente-Nunez, C. Synthetic Biology and Computer-Based Frameworks for Antimicrobial Peptide Discovery. ACS Nano. 2021;15 (2):2143-2164. doi: 10.1021/acsnano.0c09509. PubMed PMID:33538585 .
  9. Pires, DP, Monteiro, R, Mil-Homens, D, Fialho, A, Lu, TK, Azeredo, J. Designing P. aeruginosa synthetic phages with reduced genomes. Sci Rep. 2021;11 (1):2164. doi: 10.1038/s41598-021-81580-2. PubMed PMID:33495501 PubMed Central PMC7835345.
  10. Torres, MDT, Voskian, S, Brown, P, Liu, A, Lu, TK, Hatton, TA, de la Fuente-Nunez, C. Coatable and Resistance-Proof Ionic Liquid for Pathogen Eradication. ACS Nano. 2021;15 (1):966-978. doi: 10.1021/acsnano.0c07642. PubMed PMID:33438392 .
  11. Gilbert, C, Tang, TC, Ott, W, Dorr, BA, Shaw, WM, Sun, GL, Lu, TK, Ellis, T. Living materials with programmable functionalities grown from engineered microbial co-cultures. Nat Mater. 2021;20 (5):691-700. doi: 10.1038/s41563-020-00857-5. PubMed PMID:33432140 .
  12. Lenneman, BR, Fernbach, J, Loessner, MJ, Lu, TK, Kilcher, S. Enhancing phage therapy through synthetic biology and genome engineering. Curr Opin Biotechnol. 2021;68 :151-159. doi: 10.1016/j.copbio.2020.11.003. PubMed PMID:33310655 .
  13. Silva, ON, Torres, MDT, Cao, J, Alves, ESF, Rodrigues, LV, Resende, JM, Lião, LM, Porto, WF, Fensterseifer, ICM, Lu, TK, Franco, OL, de la Fuente-Nunez, C. Repurposing a peptide toxin from wasp venom into antiinfectives with dual antimicrobial and immunomodulatory properties. Proc Natl Acad Sci U S A. 2020;117 (43):26936-26945. doi: 10.1073/pnas.2012379117. PubMed PMID:33046640 PubMed Central PMC7604428.
  14. Valeri, JA, Collins, KM, Ramesh, P, Alcantar, MA, Lepe, BA, Lu, TK, Camacho, DM. Sequence-to-function deep learning frameworks for engineered riboregulators. Nat Commun. 2020;11 (1):5058. doi: 10.1038/s41467-020-18676-2. PubMed PMID:33028819 PubMed Central PMC7541510.
  15. Lai, Y, Babunovic, GH, Cui, L, Dedon, PC, Doench, JG, Fortune, SM, Lu, TK. Illuminating Host-Mycobacterial Interactions with Genome-wide CRISPR Knockout and CRISPRi Screens. Cell Syst. 2020;11 (3):239-251.e7. doi: 10.1016/j.cels.2020.08.010. PubMed PMID:32970993 .
  16. Inda, ME, Lu, TK. Microbes as Biosensors. Annu Rev Microbiol. 2020;74 :337-359. doi: 10.1146/annurev-micro-022620-081059. PubMed PMID:32660390 .
  17. Chen, CH, Lu, TK. Development and Challenges of Antimicrobial Peptides for Therapeutic Applications. Antibiotics (Basel). 2020;9 (1):. doi: 10.3390/antibiotics9010024. PubMed PMID:31941022 PubMed Central PMC7168295.
  18. Higashikuni, Y, Lu, TK. Advancing CRISPR-Based Programmable Platforms beyond Genome Editing in Mammalian Cells. ACS Synth Biol. 2019;8 (12):2607-2619. doi: 10.1021/acssynbio.9b00297. PubMed PMID:31751114 .
  19. Inda, ME, Broset, E, Lu, TK, de la Fuente-Nunez, C. Emerging Frontiers in Microbiome Engineering. Trends Immunol. 2019;40 (10):952-973. doi: 10.1016/j.it.2019.08.007. PubMed PMID:31601521 .
  20. Yehl, K, Lemire, S, Yang, AC, Ando, H, Mimee, M, Torres, MT, de la Fuente-Nunez, C, Lu, TK. Engineering Phage Host-Range and Suppressing Bacterial Resistance through Phage Tail Fiber Mutagenesis. Cell. 2019;179 (2):459-469.e9. doi: 10.1016/j.cell.2019.09.015. PubMed PMID:31585083 PubMed Central PMC6924272.
  21. Lu, Timothy K