Resources
Construct maps for plasmids described in our most recent paper
- Construct 1 – CMVp-dCAS9-VP64
- Construct 2 – PGK1p-Csy4-pA
- Construct 3 – CMVp-mKate2-Triplex-28-gRNA1-28-pA
- Construct 4 – CMVp-mKate2_EX1-[28-gRNA1-28]HSV1-mKate2_EX2-pA
- Construct 5 – P1-EYFP-pA
- Construct 6 – P2-ECFP-pA
- Construct 7 – U6p-gRNA1
- Construct 8 – CMVp-mKate2_EX1-[28-gRNA1-28]consensus-mKate2_EX2-pA
- Construct 9 – CMVp-mKate2_EX1-[28-gRNA1-28]snoRNA2-mKate2_EX2-pA
- Construct 10 – CMVp-mKate2_EX1-[28-gRNA1]HSV1-mKate2_EX2-pA
- Construct 11 – CMVp-mKate2_EX1-[gRNA1-28]HSV1-mKate2_EX2-pA
- Construct 12 – CMVp-mKate2_EX1-[gRNA2]consensus-mKate2_EX2-pA
- Construct 13 – CMVp-mKate2-Triplex-HHRibo-gRNA1-HDVRibo-pA
- Construct 14 – CMVp-mKate2-HHRibo-gRNA1-HDVRibo-pA
- Construct 15 – CMVp-HHRibo-gRNA1-HDVRibo-pA
- Construct 16 – CMVp-mKate2_EX1-[28-gRNA1-28]HSV1-mKate2_EX2-Triplex-28-gRNA2-28-pA
- Construct 17 – CMVp-mKate2-Triplex-28-gRNA1-28-gRNA2-28-pA
- Construct 18 – P1-EYFP-Triplex-28-gRNA2-28-pA
- Construct 19 – CMVp-mKate2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28
- Construct 20 – CMVp-mKate2_EX1-[miRNA]-mKate2_EX2-Triplex-28-gRNA1-28-pA
- Construct 21 – CMVp-mKate2_EX1-[miRNA]-mKate2_EX2-Triplex-28-gRNA1-28-4xmiRNA-BS-pA
- Construct 22 – CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA
- Construct 23 – CMVp-mKate2-Triplex-28-gRNA3-28
- Construct 24 – CMVp-mKate2-Triplex-28-gRNA4-28
- Construct 25 – CMVp-mKate2-Triplex-28-gRNA5-28
- Construct 26 – CMVp-mKate2-Triplex-28-gRNA6-28
- Construct 27 – P2_EYFP-pA
- SPM1128 – CMVp-dsRed2-Triplex-28-gRNA1-28-pA
- SPM1129 – CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA
- SPM1130 – CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA
Instructions for cloning in a gRNA with the sequence – GTAAGTCGGAGTACTGTCCT in to the triplex architecture:
Instructions for cloning in four multiplexed gRNAs with the following sequences –
GTGTACTCTCTGAGGTGCTC
GACGCAGATAAGAACCAGTT
GCATCAAGTCAGCCATCAGC
GGAGTCACCCTCCTGGAAAC
Instructions for cloning in a gRNA with the sequence – GTAAGTCGGAGTACTGTCCT in to the Ribozyme architecture:
