Physical Optics and Electronics Group
Prof. Rajeev Ram

Major Research Themes:

Integrated Biosystems and Biosensing


Develop advanced instrumentation for enhancing both basic research in cellular and molecular biology and the biological production of therapeutics and small molecules.

Facilities

Complete in-house microfluidic device fabrication capability. Mode-locked lasers and spectrometers ranging from UV to IR with equipment for quantitative Raman analysis.

Team

Publications

  1. Singh, G.P., S. Goh, M. Canzoneri and R. J. Ram. “Raman Spectroscopy of Complex Defined Media: Biopharmaceutical Applications.”, Journal of Raman Spectroscopy, 31Mar2015, DOI: 10.1002/jrs.4686. [PDF]
  2. Han, N., O. Purcell, K. S. Lee, T. K. Lu, and R. J. Ram . “Microfluidics for Control in Synthetic Biology.” MicroTAS, San Antonio, TX, October 2014. [PDF]
  3. Bower, D. M., K. S. Lee, R. J. Ram, and K. L. J. Prather. “Fed-Batch Microbioreactor Platform for Scale Down and Analysis of a Plasmid DNA Production Process.” Biotechnol. Bioeng. 109.8 (2012): 1976-86. [PDF]
  4. Lee, K. S., and R. J. Ram. “Microfluidic Chemostat and Turbidostat with Flow Rate, Oxygen, and Temperature Control for Dynamic Continuous Culture.” Lab on a Chip 11 (2011): 1730. [PDF]
  5. Lee, K. S., P. Boccazzi, A. J. Sinskey, and R. J. Ram. “Microscale Controlled Continuous Cell Culture.” MicroTAS, Groningen, Netherlands, October 2010. [PDF]
  6. Fernandez, M., K. S. Lee, R. J. Ram, J. H. Correia, and P. M. Mendez. “Flexible PDMS-Based Dry Electrodes for Electro-Optic Acquisition of ECG Signals in Wearable Devices.” 32nd Ann. Int. Conf. of the IEEE EMBS, Buenos Aires, Argentina, August 31 – September 4, 2010. [PDF]
  7. Lee, K. S., and R. J. Ram. “Plastic-PDMS Hybrid Devices for High Pressure Hydrolytically Stable Active Microfluidics.” MicroTAS, San Diego, CA, October 2008. [PDF]
  8. Lee, K. S., and R. J. Ram. “Optical Multiplexing of Multiple Fluorescence Sensors for Compact Lab-on-a-Chip Systems.” MicroTAS, Paris, October 2007. [PDF]
  9. Lee, K. S., and R. J. Ram. “Plastic–PDMS Bonding for High Pressure Hydrolytically Stable Active Microfluidics.” Lab on a Chip 9 (2009): 1618-24. [PDF]
  10. Lee, K. S., H. L. T. Lee, and R. J. Ram. “Polymer Waveguide Backplanes for Optical Sensor Interfaces in Microfluidics.” Lab on a Chip 7 (2007): 1539-45. [PDF]
  11. Lee, K. S., H. L. T. Lee, and R. J. Ram. “Optical Multiplexing of Multiple Fluorescence Sensors For Compact Lab-on-a-Chip Systems.” MicroTAS 2007. [PDF]
  12. Lee, H. L. T., and R. J. Ram. “Integrated Fluid Injectors and Mixers for pH Control in Miniature Bioreactor Arrays.” MicroTAS, Boston, MA, 2005. [PDF]
  13. Lee, H. L. T., P. Boccazzi, N. Gorret, R. J. Ram, and A. J. Sinskey. “In situ Bioprocess Monitoring for Escherechia Coli Using Raman Spectroscopy.” Vibrational Spectroscopy 35 (2004): 131-7. [PDF]
  14. Lee, H. L. T., P. Boccazzi, R. J. Ram, and A. J. Sinskey. “Microbioreactor Arrays with Integrated Mixers and Fluid Injectors for High-Throughput Experimentation with pH and Dissolved Oxygen Control.” Lab On a Chip 6 (2006): 1229-35. [PDF]

 

Publications

  1. W. F. Herrington Jr., G. P. Singh, D. Wu, P. W. Barone, W. Hancock & R. J. Ram, "Optical Detection of Degraded Therapeutic Proteins," Scientific Reports, 8:5098 (2018) DOI: DOI:10.1038/s41598-018-23409-z [PDF]
  2. P. Perez-Pinera, N. Han, S. Cleto, J. Cao, O. Purcell, K. A. Shah, K. Lee, R. J. Ram, T. K. Lu, "Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care," Nature Communications (2016) DOI: 10.1038/ncomms12211 [PDF]
  3. NJ Mozdzierz, KR Love, KS Lee, HLT Lee, KA Shah, R. J. Ram, J.C. Love, "A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production," Lab on a Chip (2015): 15, 2918-2922, DOI: 10.1039/C5LC00443H [PDF]
  4. G. Singh, S. Goh, M. Canzoneri, R. J. Ram, "Raman Spectroscopy of Complex Defined Media: Biopharmaceutical Applications," Journal of Raman Spectroscopy (2015) DOI: 10.1002/jrs.4686 [PDF]
  5. N. Han, O. Purcell, K. S. Lee, T. K. Lu, R. J. Ram, "Microfluidics for Control in Synthetic Biology," MicroTAS, San Antonio, TX (2014) [PDF]